Tips for successful cryopreservation of primary neural stem and progenitor cells as neurospheres:

• Freeze neurospheres at an early passage number to preserve the potential of the neural stem and progenitor cells.
• Harvest neurospheres before they grow too large (100-200 μm). If neurospheres are too big (>200 μm), their survival after cryopreservation will be negatively affected.
• Freeze whole neurospheres, do not break them up by mechanical dissociation, as this will increase the number of dead cells, significantly lowering the viability of the thawed culture.
• Freeze intact neurospheres in the appropriate species-specific Complete Proliferation Medium (i.e. supplemented with , and as appropriate) containing 10% DMSO (v/v), using controlled rate freezing containers.

For a detailed protocol, please refer to our TECHNICAL BULLETIN: .

Please contact us at techsupport@stemcell.com with any questions about this procedure, or for more information on culturing in our media.