This webinar video is presented by Sutheera Ratanasirintrawoot, a PhD Candidate at the George Daley Lab and Thorsten Schlaeger Lab, Harvard Medical School. Watch this webinar and learn about serial immunofluorescence live cell imaging to follow human iPSC colonies during reprogramming process, improved markers for determining successful human iPSC reprogramming, how to distinguish reprogramming intermediates vs. bona fide iPSC colonies and defined, serum-free culture conditions which select for bona fide iPSC colonies.