EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #72092_C
糖皮质激素通路激活剂；激活糖皮质激素受体
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总览
地塞米松是一种合成的糖皮质激素，类似于天然的糖皮质激素氢化可的松。与天然氢化可的松配体相比，地塞米松对糖皮质激素受体的亲和力更高（Kd = 5 nM vs 17 nM）。
重编程
·促进小鼠胰腺细胞向肝细胞的转分化（Shen et al.）。
分化
·促进人间充质细胞向成骨、脂肪和软骨方向分化（Jaiswal et al., Mackay et al., Pittenger et al.）。
·促进小鼠间充质细胞向成骨、脂肪和软骨方向分化（Tropel et al.）。
·促进小鼠和人胚胎干细胞（ES）向成熟肝细胞分化（Cai et al., Kubo et al.）。
·促进小鼠胎肝细胞成熟（Kamiya et al.）。
别名
MK 125，NSC 34521
细胞类型
间充质干/祖细胞，胰腺细胞，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化，重编程
研究领域
上皮细胞研究，干细胞生物学
CAS 编号
50-02-2
化学式
C₂₂H₂₉FO₅
分子量
392.5 克/摩尔
纯度
≥98%
通路
糖皮质激素
靶点
糖皮质激素受体
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
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相关材料与文献
技术资料 (4)
文献 (9)
Directed differentiation of human embryonic stem cells into functional hepatic cells. Hepatology (Baltimore, Md.) 2007 MAY
Abstract
UNLABELLED The differentiation capacity of human embryonic stem cells (hESCs) holds great promise for therapeutic applications. We report a novel three-stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum-free medium. Human ESCs were first differentiated into definitive endoderm cells by 3 days of Activin A treatment. Next, the presence of fibroblast growth factor-4 and bone morphogenetic protein-2 in the culture medium for 5 days induced efficient hepatic differentiation from definitive endoderm cells. Approximately 70% of the cells expressed the hepatic marker albumin. After 10 days of further in vitro maturation, these cells expressed the adult liver cell markers tyrosine aminotransferase, tryptophan oxygenase 2, phosphoenolpyruvate carboxykinase (PEPCK), Cyp7A1, Cyp3A4 and Cyp2B6. Furthermore, these cells exhibited functions associated with mature hepatocytes including albumin secretion, glycogen storage, indocyanine green, and low-density lipoprotein uptake, and inducible cytochrome P450 activity. When transplanted into CCl4 injured severe combined immunodeficiency mice, these cells integrated into the mouse liver and expressed human alpha-1 antitrypsin for at least 2 months. In addition, we found that the hESC-derived hepatic cells were readily infected by human immunodeficiency virus-hepatitis C virus pseudotype viruses. CONCLUSION We have developed an efficient way to direct the differentiation of human embryonic stem cells into cells that exhibit characteristics of mature hepatocytes. Our studies should facilitate searching the molecular mechanisms underlying human liver development, and form the basis for hepatocyte transplantation and drug tests.
Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental cell research 2004 MAY
Abstract
The future use of adult mesenchymal stem cells (MSCs) for human therapies depends on the establishment of preclinical studies with other mammals such as mouse. Surprisingly, purification and characterisation of murine MSCs were only poorly documented. The aim of this study was to purify mouse MSCs from adult bone marrow and to functionally characterise their abilities to differentiate along diverse lineages. Adherent cells from adult C57Bl/6J mouse bone marrow were depleted of granulo-monocytic cells and subsequently allowed to grow on fibronectin-coated dishes in presence of fetal bovine serum and growth factors. The growing fibroblastoid cell population primarily consisted of spindle- and star-shaped cells with significant renewal capacity as they were cultured until 30 passages (about 60 doubling population). We fully demonstrated the MSC phenotype of these cells by inducing them to differentiate along osteoblastic, adipocytic, and chondrocytic pathways. Mouse MSCs (mMSCs) sharing the same morphological and functional characteristics as human MSCs can be successfully isolated from adult bone marrow without previous mouse or bone marrow treatment. Therefore, mMSCs will be an important tool to study the in vivo behaviour and fate of this cell type after grafting in mouse pathology models.
Development of definitive endoderm from embryonic stem cells in culture. Development (Cambridge, England) 2004 APR
Abstract
The cellular and molecular events regulating the induction and tissue-specific differentiation of endoderm are central to our understanding of the development and function of many organ systems. To define and characterize key components in this process, we have investigated the potential of embryonic stem (ES) cells to generate endoderm following their differentiation to embryoid bodies (EBs) in culture. We found that endoderm can be induced in EBs, either by limited exposure to serum or by culturing in the presence of activin A (activin) under serum-free conditions. By using an ES cell line with the green fluorescent protein (GFP) cDNA targeted to the brachyury locus, we demonstrate that endoderm develops from a brachyury(+) population that also displays mesoderm potential. Transplantation of cells generated from activin-induced brachyury(+) cells to the kidney capsule of recipient mice resulted in the development of endoderm-derived structures. These findings demonstrate that ES cells can generate endoderm in culture and, as such, establish this differentiation system as a unique murine model for studying the development and specification of this germ layer.
更多信息
| Molecular Weight | 392.5 g/mol |
|---|---|
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
| Alternative Names | MK 125, NSC 34521 |
| Cas Number | 50-02-2 |
| Chemical Formula | C₂₂H₂₉FO₅ |
| 纯度 | ≥ 98% |
| Target | Glucocorticoid Receptor |
| Pathway | Glucocorticoid |
相关产品
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STEMdiff™ Definitive Endoderm KitDefined animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
