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EasySep™ Direct人Naïve B细胞分选试剂盒

全血免疫磁珠负选试剂盒
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产品号 #（选择产品）

产品号 #19264_C

全血免疫磁珠负选试剂盒

产品优势

  • > 99.9%的红细胞（RBC）去除率，无需密度梯度离心、沉降或裂解
  • 操作简单、快捷，且无需分离柱
  • 分选获得的细胞纯度高达95%
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™ Direct人Naïve B细胞分选试剂盒（产品号#19264）
    • EasySep™ Direct人Naïve B细胞分选抗体混合物，2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™磁珠，4 x 2.5 mL
main product photo
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

该试剂盒用于直接从人全血中分离高纯度的Naïve B细胞。分选后的细胞未被抗体或磁珠标记，并且仅经过最少的操作处理。

该试剂盒通过识别特异性细胞表面标志的抗体来去除非Naïve B细胞。非目的细胞会被抗体和EasySep™ Direct RapidSpheres™磁珠标记，并使用EasySep™磁极完成分选。目的细胞可被直接收集到新的管中，立即用于下游应用，例如流式细胞术、培养或DNA/RNA 提取。

该试剂盒通过识别特异性细胞表面标志的抗体来去除非Naïve B细胞。非目的细胞会被抗体和EasySep™ Direct RapidSpheres™磁珠标记，并使用EasySep™磁极完成分选。目的细胞可被直接收集到新的管中，立即用于下游应用，例如流式细胞术、培养或DNA/RNA 提取。

该试剂盒通过识别特异性细胞表面标志的抗体来去除非Naïve B细胞。非目的细胞会被抗体和EasySep™ Direct RapidSpheres™磁珠标记，并使用EasySep™磁极完成分选。目的细胞可被直接收集到新的管中，立即用于下游应用，例如流式细胞术、培养或DNA/RNA 提取。

该试剂盒通过识别特异性细胞表面标志的抗体来去除非Naïve B细胞。非目的细胞会被抗体和EasySep™ Direct RapidSpheres™磁珠标记，并使用EasySep™磁极完成分选。目的细胞可被直接收集到新的管中，立即用于下游应用，例如流式细胞术、培养或DNA/RNA 提取。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞
 
种属
人
 
样本来源
Whole Blood
 
筛选方法
Negative
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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实验数据

Starting with human whole blood from normal healthy donors, the typical naïve B cell (CD19+CD27-) content of the non-lysed final isolated fraction is 918 ± 36% (gated on CD45) or 824 ± 126% (not gated on CD45)

Figure 1. Typical EasySep™ Direct Human Naïve B Cell Isolation Profile

Starting with human whole blood from normal healthy donors, the typical naïve B cell (CD19+CD27-) content of the non-lysed final isolated fraction is 91.8 ± 3.6% (gated on CD45) or 82.4 ± 12.6% (not gated on CD45). In the above example, the naïve B cell (CD19+CD27-) content of the lysed whole blood start sample and non-lysed final isolated fraction is 5.1% and 91.5% (gated on CD45), respectively, or 5.1% and 90.0% (not gated on CD45), respectively. The starting frequency of naïve B cells in the non-lysed whole blood start sample above is 0.009% (data not shown).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Direct Human Naïve B Cell Isolation Kit
Catalog #
19264
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Direct Human Naïve B Cell Isolation Kit
Catalog #
19264
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Direct Human Naïve B Cell Isolation Kit
Catalog #
19264
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

相关材料与文献

技术资料 (12)

EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes
Brochure
EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human B Cell Isolation Product Selection
Brochure
Human B Cell Isolation Product Selection
High-Throughput Immunodensity-Based Cell Subset Isolation Directly from Whole Blood
Technical Bulletin
High-Throughput Immunodensity-Based Cell Subset Isolation Directly from Whole Blood
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protocol
5:17
Video
How to Isolate Cells Directly from Whole Blood Using the Easy 50 EasySep™ Magnet: 30-Minute Protoc...
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet
6:13
Video
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets
4:30
Video
How to Isolate Cells Directly from Whole Blood Using the EasySep™ Purple/Silver Magnets

常见问题

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

文献 (1)

BCR-associated factors driving chronic lymphocytic leukemia cells proliferation ex vivo. C. Schleiss et al. Scientific reports 2019 jan

Abstract

A chronic antigenic stimulation is believed to sustain the leukemogenic development of chronic lymphocytic leukemia (CLL) and most of lymphoproliferative malignancies developed from mature B cells. Reproducing a proliferative stimulation ex vivo is critical to decipher the mechanisms of leukemogenesis in these malignancies. However, functional studies of CLL cells remains limited since current ex vivo B cell receptor (BCR) stimulation protocols are not sufficient to induce the proliferation of these cells, pointing out the need of mandatory BCR co-factors in this process. Here, we investigated benefits of several BCR co-stimulatory molecules (IL-2, IL-4, IL-15, IL-21 and CD40 ligand) in multiple culture conditions. Our results demonstrated that BCR engagement (anti-IgM ligation) concomitant to CD40 ligand, IL-4 and IL-21 stimulation allowed CLL cells proliferation ex vivo. In addition, we established a proliferative advantage for ZAP70 positive CLL cells, associated to an increased phosphorylation of ZAP70/SYK and STAT6. Moreover, the use of a tri-dimensional matrix of methylcellulose and the addition of TLR9 agonists further increased this proliferative response. This ex vivo model of BCR stimulation with T-derived cytokines is a relevant and efficient model for functional studies of CLL as well as lymphoproliferative malignancies.
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更多信息

更多信息
种属 Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • EasyEights™ EasySep™ Magnet (Catalog #18103)
样本来源 Whole Blood
Selection Method Negative
标记抗体
  1. 抗人CD45抗体，克隆HI30
    抗人CD45抗体，克隆HI30

    小鼠单克隆IgG1抗体，抗人、黑猩猩CD45

  2. 抗人CD19抗体，克隆HIB19
    抗人CD19抗体，克隆HIB19

    抗人、黑猩猩CD19的小鼠单克隆IgG1抗体

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