总览

使用简单的两步法从新鲜的全脐带血中分离高纯度的CD34+细胞。首先，使用RosetteSep™人祖细胞基础预富集抗体混合物 (产品号 #15226C) 识别T细胞、B细胞和髓系细胞表面标志物的抗体，预富集造血祖细胞。然后使用EasySep™人CD34正选抗体混合物 (产品号 #18096C) 识别CD34的抗体来分选CD34+细胞。

本试剂盒不会去除血小板。若需从可能含有血小板污染的脐带血中分离CD34+细胞，推荐使用EasySep™人类脐带血CD34阳性分选试剂盒II（目录号17896）。

从新鲜的人外周血或白膜分离CD34+细胞，推荐使用人全血CD34+细胞全套试剂盒（产品号 #15086）。

如需从其他样本分离CD34+细胞，包括新鲜或冻存的动员外周血、骨髓单个核细胞、冻存的脐带血单个核细胞，请使用EasySep™人CD34正选试剂盒II（产品号 #17856）。

磁极兼容性
• EasySep™磁极（产品号 #18000） • “The Big Easy” EasySep™磁极（产品号 #18001） • EasyEights™ EasySep™磁极（产品号 #18103） • RoboSep™-S（产品号 #21000）

分类
细胞分选试剂盒

细胞类型
造血干/祖细胞

种属
人

样本来源
脐带血

分选方法
正选

应用
细胞分选

品牌
EasySep，RoboSep，RosetteSep

研究领域
免疫，干细胞生物学

查看更多显示更少

实验数据

Figure 1. Typical EasySep™ Human Cord Blood CD34 Positive Selection III Isolation Profile

Starting with fresh cord blood, the CD34+ cell content of the isolated fraction is typically 87 ± 12% (mean ± SD, n=10, data obtained using the purple EasySep™ Magnet). The CD34+ cell content of the starting sample is typically 0.5 ± 0.25% (mean ± SD).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

RoboSep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897RF

Lot #

All

Language

English

Document Type

Product Information Sheet

Product Name

EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

RoboSep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

RoboSep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

RoboSep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 4

Product Name

RoboSep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897RF

Lot #

All

Language

English

Document Type

Safety Data Sheet 1

Product Name

EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897

Lot #

All

Language

English

Document Type

Safety Data Sheet 2

Product Name

EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897

Lot #

All

Language

English

Document Type

Safety Data Sheet 3

Product Name

EasySep™ Human Cord Blood CD34 Positive Selection Kit III

Catalog #

17897

Lot #

All

Language

English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area

Workflow Stages

Workflow Stages for Hematopoietic Stem and Progenitor Cell Research

Cell Sourcing and Isolation

Expansion and Differentiation

Analysis

What is RosetteSep™?

RosetteSep™ is a rapid cell separation procedure for the isolation of purified cells directly from whole blood, without columns or magnets.

How does RosetteSep™ work?

The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. The unwanted cells then pellet with the free RBCs when centrifuged over a density centrifugation medium (e.g. Ficoll-Paque™ PLUS, Lymphoprep™).

What factors affect cell recovery?

The temperature of the reagents can affect cell recovery. All reagents should be at room temperature (sample, density centrifugation medium, PBS, centrifuge) before performing the isolations. Layering can also affect recovery so be sure to carefully layer the sample to avoid mixing with the density centrifugation medium as much as possible. Be sure to collect the entire enriched culture without disturbing the RBC pellet. A small amount of density centrifugation medium can be collected without worry.

Which cell samples can RosetteSep™ be used with?

RosetteSep™ can be used with leukapheresis samples, bone marrow or buffy coat, as long as: the concentration of cells does not exceed 5 x 10⁷ per mL (can dilute if necessary); and there are at least 100 RBCs for every nucleated cell (RBCs can be added if necessary).

Can RosetteSep™ be used with previously frozen or cultured cells?

Yes. Cells should be re-suspended at 2 - 5 x 10⁷ cells / mL in PBS + 2% FBS. Fresh whole blood should be added at 250 µL per mL of sample, as a source of red cells.

Can RosetteSep™ be used to enrich progenitors from cord blood?

Yes. Sometimes cord blood contains immature nucleated red cells that have a lower density than mature RBCs. These immature red cells do not pellet over Ficoll™, which can lead to a higher RBC contamination than peripheral blood separations.

Does RosetteSep™ work with mouse cells?

No, but we have developed EasySep™, a magnetic-based cell isolation system which works with mouse and other non-human species.

Which anticoagulant should be used with RosetteSep™?

Peripheral blood should be collected in heparinized Vacutainers. Cord blood should be collected in ACD.

Should the anticoagulant be washed off before using RosetteSep™?

No, the antibody cocktail can be added directly to the sample.

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.

EasySep™人脐带血CD34正选试剂盒III

产品号 #17897

产品号 #17897RF

产品号 #（选择产品）

产品号 #17897_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

Request Pricing

更多信息

Scientists Helping Scientists™

种属	Human
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™ (Catalog #21000)
样本来源	Cord Blood
Selection Method	Positive

EasySep™人脐带血CD34正选试剂盒III

产品号 #17897

产品号 #17897RF

产品号 #（选择产品）

产品号 #17897_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

应用领域

相关材料与文献

技术资料 (9)

常见问题

What is RosetteSep™?

How does RosetteSep™ work?

What factors affect cell recovery?

Which cell samples can RosetteSep™ be used with?

Can RosetteSep™ be used with previously frozen or cultured cells?

Can RosetteSep™ be used to enrich progenitors from cord blood?

Does RosetteSep™ work with mouse cells?

Which anticoagulant should be used with RosetteSep™?

Should the anticoagulant be washed off before using RosetteSep™?

Can EasySep™ be used for either positive or negative selection?

How does the separation work?

Which columns do I use?

How can I analyze the purity of my enriched sample?

Can EasySep™ separations be automated?

Can EasySep™ be used to isolate rare cells?

Are the EasySep™ magnetic particles FACS-compatible?

Can the EasySep™ magnetic particles be removed after enrichment?

Can I alter the separation time in the magnet?

For positive selection, can I perform more than 3 separations to increase purity?

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Request Pricing

更多信息

欢迎您关注STEMCELL Technologies的微信公众平台