EasySep™小鼠FITC正选试剂盒II

对标记 FITC 偶联抗体的小鼠细胞进行免疫磁珠正选分离

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产品号 #（选择产品）

产品号 #17668_C

用于免疫磁珠正选细胞的试剂盒

产品优势

快速、简单
无需分离柱

产品组分包括

EasySep™小鼠FITC正选试剂盒II（产品号 #17668）

EasySep™ FITC分选抗体混合物，1 mL
小鼠FcR阻断剂，0.1 mL
EasySep™ Dextran RapidSpheres™磁珠，1 mL
RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支

EasySep™小鼠FITC正选试剂盒II（产品号 #17668）

EasySep™ FITC分选抗体混合物，1 mL
小鼠FcR阻断剂，0.1 mL
EasySep™ Dextran RapidSpheres™磁珠，1 mL
RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支
RoboSep™ 缓冲液（产品号 #20104）x 2
RoboSep™过滤吸头（产品号 #20125）x 2

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总览

使用EasySep™小鼠FITC正选试剂盒II，通过免疫磁珠正选法可从小鼠脾细胞、骨髓或其他组织的单细胞悬液中分离经异硫氰酸荧光素（FITC）偶联抗体标记的高纯度小鼠细胞。EasySep™技术已在超过20年的发表研究中得到广泛应用，结合单克隆抗体特异性与无柱磁珠分选系统的简便性。

在此正选步骤中，目标细胞被能够识别FITC和磁珠的抗体复合物所标记。本试剂盒还含抗小鼠Fc受体阻断剂，以防止非特异性结合。标记细胞经EasySep™磁极分离后，未标记细胞被去除，FITC阳性细胞保留在试管中。分选后的细胞可直接用于流式细胞术、培养或细胞检测。

了解更多EasySep™免疫磁珠技术或RoboSep™自动化细胞分选系统。

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

技术资料 (6)

Accumulation of ?? T cells in visceral fat with aging promotes chronic inflammation. M. E. C. Bruno et al. GeroScience 2022 jun

Abstract

Adipose tissue dysfunction is strongly linked to the development of chronic inflammation and cardiometabolic disorders in aging. While much attention has been given to the role of resident adipose tissue immune cells in the disruption of homeostasis in obesity, age-specific effects remain understudied. Here, we identified and characterized a population of ?? T cells, which show unique age-dependent accumulation in the visceral adipose tissue (VAT) of both mice and humans. Diet-induced obesity likewise increased ?? T cell numbers; however, the effect was greater in the aged where the increase was independent of fat mass. ?? T cells in VAT express a tissue-resident memory T cell phenotype (CD44hiCD62LlowCD69+) and are predominantly IL-17A-producing cells. Transcriptome analyses of immunomagnetically purified ?? T cells identified significant age-associated differences in expression of genes related to inflammation, immune cell composition, and adipocyte differentiation, suggesting age-dependent qualitative changes in addition to the quantitative increase. Genetic deficiency of ?? T cells in old age improved the metabolic phenotype, characterized by increased respiratory exchange ratio, and lowered levels of IL-6 both systemically and locally in VAT. Decreased IL-6 was predominantly due to reduced production by non-immune stromal cells, primarily preadipocytes, and adipose-derived stem cells. Collectively, these findings suggest that an age-dependent increase of tissue-resident ?? T cells in VAT contributes to local and systemic chronic inflammation and metabolic dysfunction in aging.

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Combinatorial depletions of G-protein coupled receptor kinases in immune cells identify pleiotropic and cell type-specific functions. K. M. Glaser et al. Frontiers in immunology 2022

Abstract

G-protein coupled receptor kinases (GRKs) participate in the regulation of chemokine receptors by mediating receptor desensitization. They can be recruited to agonist-activated G-protein coupled receptors (GPCRs) and phosphorylate their intracellular parts, which eventually blocks signal propagation and often induces receptor internalization. However, there is growing evidence that GRKs can also control cellular functions beyond GPCR regulation. Immune cells commonly express two to four members of the GRK family (GRK2, GRK3, GRK5, GRK6) simultaneously, but we have very limited knowledge about their interplay in primary immune cells. In particular, we are missing comprehensive studies comparing the role of this GRK interplay for (a) multiple GPCRs within one leukocyte type, and (b) one specific GPCR between several immune cell subsets. To address this issue, we generated mouse models of single, combinatorial and complete GRK knockouts in four primary immune cell types (neutrophils, T cells, B cells and dendritic cells) and systematically addressed the functional consequences on GPCR-controlled cell migration and tissue localization. Our study shows that combinatorial depletions of GRKs have pleiotropic and cell-type specific effects in leukocytes, many of which could not be predicted. Neutrophils lacking all four GRK family members show increased chemotactic migration responses to a wide range of GPCR ligands, whereas combinatorial GRK depletions in other immune cell types lead to pro- and anti-migratory responses. Combined depletion of GRK2 and GRK6 in T cells and B cells shows distinct functional outcomes for (a) one GPCR type in different cell types, and (b) different GPCRs in one cell type. These GPCR-type and cell-type specific effects reflect in altered lymphocyte chemotaxis in vitro and localization in vivo. Lastly, we provide evidence that complete GRK deficiency impairs dendritic cell homeostasis, which unexpectedly results from defective dendritic cell differentiation and maturation in vitro and in vivo. Together, our findings demonstrate the complexity of GRK functions in immune cells, which go beyond GPCR desensitization in specific leukocyte types. Furthermore, they highlight the need for studying GRK functions in primary immune cells to address their specific roles in each leukocyte subset.

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物种	小鼠
Magnet Compatibility	• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
样本来源	其它细胞系, 脾脏, 骨髓
Selection Method	Positive

EasySep™小鼠FITC正选试剂盒II

产品号 #17668

产品号 #17668RF

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产品号 #17668_C

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EasySep™小鼠FITC正选试剂盒II

产品号 #17668

产品号 #17668RF

产品号 #（选择产品）

产品号 #17668_C

产品优势

产品组分包括

总览

实验数据

产品说明书及文档

相关材料与文献

技术资料 (6)

文献 (2)

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