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EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒

免疫磁珠去除生物素化抗体标记的单一或多种小鼠非目的细胞类型
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产品号 #（选择产品）

产品号 #19860_C

免疫磁珠去除生物素化抗体标记的单一或多种小鼠非目的细胞类型

产品优势

  • 快速、简单
  • 无需分离柱
  • 获得的活细胞无标记

产品组分包括

  • EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒（产品号 #19860）
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
    • EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL
  • RoboSep™小鼠Streptavidin RapidSpheres™分选试剂盒（产品号 #19860RF）
    • EasySep™ Streptavidin RapidSpheres™ 50001磁珠，1 mL
    • EasySep™小鼠FcR阻断剂（产品号 #18731），0.5 mL
    • RoboSep™空管
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™ 过滤吸头（产品号 #20125）
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

EasySep™小鼠Streptavidin RapidSpheres™分选试剂盒，通过免疫磁珠负选，可高效去除小鼠脾细胞或其他组织样本中被生物素化抗体标记的单一或多种非目的细胞类型。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

该优化的简易EasySep™流程，通过用生物素化抗体和链霉亲和素包被的磁珠（Streptavidin RapidSpheres™磁珠）标记非目的细胞。通过EasySep™磁极分离被标记的细胞，未标记的目的细胞被简单地倾倒出。分选后的细胞可立即用于下游应用，例如流式细胞术、培养或 DNA/RNA 提取。

不建议使用该试剂盒对小鼠细胞进行正选。如需正选，请使用EasySep™小鼠生物素正选试剂盒II（产品号 #17665）。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、补充剂、抗体等。

该优化的简易EasySep™流程，通过用生物素化抗体和链霉亲和素包被的磁珠（Streptavidin RapidSpheres™磁珠）标记非目的细胞。通过EasySep™磁极分离被标记的细胞，未标记的目的细胞被简单地倾倒出。分选后的细胞可立即用于下游应用，例如流式细胞术、培养或 DNA/RNA 提取。

不建议使用该试剂盒对小鼠细胞进行正选。如需正选，请使用EasySep™小鼠生物素正选试剂盒II（产品号 #17665）。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、补充剂、抗体等。

该优化的简易EasySep™流程，通过用生物素化抗体和链霉亲和素包被的磁珠（Streptavidin RapidSpheres™磁珠）标记非目的细胞。通过EasySep™磁极分离被标记的细胞，未标记的目的细胞被简单地倾倒出。分选后的细胞可立即用于下游应用，例如流式细胞术、培养或 DNA/RNA 提取。

不建议使用该试剂盒对小鼠细胞进行正选。如需正选，请使用EasySep™小鼠生物素正选试剂盒II（产品号 #17665）。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、补充剂、抗体等。

该优化的简易EasySep™流程，通过用生物素化抗体和链霉亲和素包被的磁珠（Streptavidin RapidSpheres™磁珠）标记非目的细胞。通过EasySep™磁极分离被标记的细胞，未标记的目的细胞被简单地倾倒出。分选后的细胞可立即用于下游应用，例如流式细胞术、培养或 DNA/RNA 提取。

不建议使用该试剂盒对小鼠细胞进行正选。如需正选，请使用EasySep™小鼠生物素正选试剂盒II（产品号 #17665）。

了解更多关于免疫磁珠EasySep™技术的工作原理，或如何通过RoboSep™实现免疫磁珠细胞分选全自动化。探索为您的实验流程优化的更多产品，包括培养基、补充剂、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
小鼠
 
样本来源
其它细胞系，Spleen
 
筛选方法
删除，Negative
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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实验数据

Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Figure 1. Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Figure 2. Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

Figure 3. Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
1000147071 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
1000147072 or higher
Language
English
Document Type
Product Information Sheet 1
Product Name
EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860
Lot #
1000147071 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860
Lot #
1000147072 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit
Catalog #
19860
Lot #
All
Language
English

相关材料与文献

技术资料 (7)

Mouse Cell Isolation
Brochure
Mouse Cell Isolation
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Systemic Dysfunction and Plasticity of the Immune Macroenvironment in Cancer Models
44:55
Webinar
Systemic Dysfunction and Plasticity of the Immune Macroenvironment in Cancer Models

文献 (9)

Thymocyte Maturation and Emigration in Adult Mice. K. Joannou et al. Journal of immunology (Baltimore, Md. : 1950) 2022 may

Abstract

Several unique waves of ?? T cells are generated solely in the fetal/neonatal thymus, whereas additional ?? T cell subsets are generated in adults. One intriguing feature of ?? T cell development is the coordination of differentiation and acquisition of effector function within the fetal thymus; however, it is less clear whether this paradigm holds true in adult animals. In this study, we investigated the relationship between maturation and thymic export of adult-derived ?? thymocytes in mice. In the Rag2pGFP model, immature (CD24+) ?? thymocytes expressed high levels of GFP whereas only a minority of mature (CD24-) ?? thymocytes were GFP+ Similarly, most peripheral GFP+ ?? T cells were immature. Analysis of ?? recent thymic emigrants (RTEs) indicated that most ?? T cell RTEs were CD24+ and GFP+, and adoptive transfer experiments demonstrated that immature ?? thymocytes can mature outside the thymus. Mature ?? T cells largely did not recirculate to the thymus from the periphery; rather, a population of mature ?? thymocytes that produced IFN-? or IL-17 remained resident in the thymus for at least 60 d. These data support the existence of two populations of ?? T cell RTEs in adult mice: a majority subset that is immature and matures in the periphery after thymic emigration, and a minority subset that completes maturation within the thymus prior to emigration. Additionally, we identified a heterogeneous population of resident ?? thymocytes of unknown functional importance. Collectively, these data shed light on the generation of the ?? T cell compartment in adult mice.
View publication
Cutting Edge: Enhanced Antitumor Immunity in ST8Sia6 Knockout Mice. D. J. Friedman et al. Journal of immunology (Baltimore, Md. : 1950) 2022 apr

Abstract

Inhibitory receptors have a critical role in the regulation of immunity. Siglecs are a family of primarily inhibitory receptors expressed by immune cells that recognize specific sialic acid modifications on cell surface glycans. Many tumors have increased sialic acid incorporation. Overexpression of the sialyltransferase ST8Sia6 on tumors led to altered immune responses and increased tumor growth. In this study, we examined the role of ST8Sia6 on immune cells in regulating antitumor immunity. ST8Sia6 knockout mice had an enhanced immune response to tumors. The loss of ST8Sia6 promoted an enhanced intratumoral activation of macrophages and dendritic cells, including upregulation of CD40. Intratumoral regulatory T cells exhibited a more inflammatory phenotype in ST8Sia6 knockout mice. Using adoptive transfer studies, the change in regulatory T cell phenotype was not cell intrinsic and depended on the loss of ST8Sia6 expression in APCs. Thus, ST8Sia6 generates ligands for Siglecs that dampen antitumor immunity.
View publication
Repurposing a novel anti-cancer RXR agonist to attenuate acute GVHD and maintain graft-versus-leukemia responses. G. Thangavelu et al. Blood 2020 sep

Abstract

The nuclear receptors (NR) retinoid X receptors (RXRs) exert immunomodulatory functions to control inflammation and metabolism via homodimers and heterodimers with several other NRs including retinoic acid receptors. IRX4204 is a novel, highly specific RXR agonist in clinical trials that potently and selectively activates RXR homodimers but not heterodimers. Here, we show that in vivo IRX4204 was compared favorably with FK506 in abrogating acute graft-versus-host disease (GVHD), which was associated with inhibiting allogeneic donor T cell proliferation, reducing T helper 1 differentiation and promoting regulatory T cell (Treg) generation. Recipient IRX4204 treatment reduced intestinal injury and decreased IFN-$\gamma$ and TNF-$\alpha$ serum levels. Transcriptional analysis of donor T cells isolated from intestines of GVHD mice treated with IRX4204 revealed significant decreases in transcripts regulating pro-inflammatory pathways. In vitro, inducible Treg differentiation from na{\{i}}ve CD4+ T cells was enhanced by IRX4204; in vivo IRX4204 increased the conversion of donor Foxp3- T cells into peripheral Foxp3+ Tregs in GVHD mice. Using Foxp3 lineage tracer mice in which both the origin and current FoxP3 expression of Tregs can be tracked we demonstrate that IRX4204 supported Treg stability. Despite favoring Tregs and reducing Th1 differentiation IRX4204-treated recipients maintained graft-versus-leukemia responses against both leukemia and lymphoma cells. Notably IRX4204 reduced in vitro human T cell proliferation and enhanced Treg generation in mixed lymphocyte reaction cultures. Collectively these beneficial effects indicate that targeting RXRs with IRX4204 could be used as a novel approach to prevent acute GVHD in the clinic."
View publication
View All Publications

更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • RoboSep™-S (Catalog #21000)
样本来源 Other, Spleen
Selection Method Depletion, Negative
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