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EasySep™ Release 小鼠APC正选试剂盒

采用可解离磁珠技术的免疫磁珠正选试剂盒
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产品号 #（选择产品）

产品号 #100-0033_C

采用可解离磁珠技术的免疫磁珠正选试剂盒

产品优势

  • 在40分钟内从小鼠组织中分选出用APC偶联抗体标记的高纯度细胞
  • 无需清洗去除EasySep™ Releasable RapidSpheres™可解离磁珠

产品组分包括

  • EasySep™ Release 小鼠APC正选试剂盒（产品号 #100-0033）
    • EasySep™ Release APC正选抗体混合物，1 mL
    • EasySep™ Releasable RapidSpheres™磁珠，1 mL
    • EasySep™Release 缓冲液（浓缩），3 x 1 mL
    • 小鼠FcR阻断剂（产品号 #18731），5 mL
main product photo
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.
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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

总览
实验数据
产品说明书及文档
相关材料与文献
相关产品

总览

EasySep™ Release 小鼠 APC 正选试剂盒为通过免疫磁珠正选从小鼠组织中分选出标记APC偶联抗体的细胞而设计。

目的细胞用抗体和磁珠标记，并通过 EasySep™ 磁极进行无柱分选。非目的细胞通过简单倾倒弃去，而目的细胞则保留在试管中。随后，结合在使用EasySep™分选的APC偶联抗体标记的细胞上的磁珠被解离，目的细胞可立即用于下游应用。使用该EasySep™ Release 试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。

目的细胞用抗体和磁珠标记，并通过 EasySep™ 磁极进行无柱分选。非目的细胞通过简单倾倒弃去，而目的细胞则保留在试管中。随后，结合在使用EasySep™分选的APC偶联抗体标记的细胞上的磁珠被解离，目的细胞可立即用于下游应用。使用该EasySep™ Release 试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。

目的细胞用抗体和磁珠标记，并通过 EasySep™ 磁极进行无柱分选。非目的细胞通过简单倾倒弃去，而目的细胞则保留在试管中。随后，结合在使用EasySep™分选的APC偶联抗体标记的细胞上的磁珠被解离，目的细胞可立即用于下游应用。使用该EasySep™ Release 试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。

目的细胞用抗体和磁珠标记，并通过 EasySep™ 磁极进行无柱分选。非目的细胞通过简单倾倒弃去，而目的细胞则保留在试管中。随后，结合在使用EasySep™分选的APC偶联抗体标记的细胞上的磁珠被解离，目的细胞可立即用于下游应用。使用该EasySep™ Release 试剂盒分选之后，细胞表面仍结合有抗体复合物，并可能与Brilliant Violet™偶联的抗体、聚乙二醇修饰的蛋白质或其他化学相关配体相互作用。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102)
 
亚型
细胞分选试剂盒
 
细胞类型
其它细胞系
 
种属
小鼠
 
样本来源
Bone Marrow，其它细胞系，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫
 

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实验数据

Using the EasySep™ Release Mouse APC Positive Selection Kit, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Mouse APC Positive Selection Kit

In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 64.8% and 98.5%, respectively.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
1000157896 or lower
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
1000157897 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Release Mouse APC Positive Selection Kit
Catalog #
100-0033
Lot #
All
Language
English

相关材料与文献

技术资料 (4)

Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres�...
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Cells from Complex Tissues in Less Than 30 Minutes
Scientific Poster
Releasable RapidSpheres Enable Immunomagnetic Purification of Highly Viable and Functional Immune Ce...
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable RapidSpheres™ Results in Functional, Pure and Particle-Free Cells
Scientific Poster
Fast and Easy Immunomagnetic Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells w...

文献 (1)

Short palate, lung, and nasal epithelial clone 1 (SPLUNC1) level determines steroid-resistant airway inflammation in aging. A. K. Jaiswal et al. American journal of physiology. Lung cellular and molecular physiology 2022 jan

Abstract

Asthma and its heterogeneity change with age. Increased airspace neutrophil numbers contribute to severe steroid-resistant asthma exacerbation in the elderly, which correlates with the changes seen in adults with asthma. However, whether that resembles the same disease mechanism and pathophysiology in aged and adults is poorly understood. Here, we sought to address the underlying molecular mechanism of steroid-resistant airway inflammation development and response to corticosteroid (Dex) therapy in aged mice. To study the changes in inflammatory mechanism, we used a clinically relevant treatment model of house-dust mite (HDM)-induced allergic asthma and investigated lung adaptive immune response in adult (20-22 wk old) and aged (80-82 wk old) mice. Our result indicates an age-dependent increase in airway hyperresponsiveness (AHR), mixed granulomatous airway inflammation comprising eosinophils and neutrophils, and Th1/Th17 immune response with progressive decrease in frequencies and numbers of HDM-bearing dendritic cells (DC) accumulation in the draining lymph node (DLn) of aged mice as compared with adult mice. RNA-Seq experiments of the aged lung revealed short palate, lung, and nasal epithelial clone 1 (SPLUNC1) as one of the steroid-responsive genes, which progressively declined with age and further by HDM-induced inflammation. Moreover, we found increased glycolytic reprogramming, maturation/activation of DCs, the proliferation of OT-II cells, and Th2 cytokine secretion with recombinant SPLUNC1 (rSPLUNC1) treatment. Our results indicate a novel immunomodulatory role of SPLUNC1 regulating metabolic adaptation/maturation of DC. An age-dependent decline in the SPLUNC1 level may be involved in developing steroid-resistant airway inflammation and asthma heterogeneity.
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更多信息

更多信息
种属 Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • EasyPlate™ EasySep™ Magnet (Catalog #18102)
样本来源 Bone Marrow, Other, Spleen
Selection Method Positive
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