EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #05601_C
用于 CFU 检测人乳腺上皮细胞的培养
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专为您的实验方案打造的产品
要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。
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Collagen SolutionFor preparation of collagen gels and for coating cell culture surfaces
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Ammonium Chloride SolutionReagent for lysis of red blood cells
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Dispase (5 U/mL)5 U/mL dispase in Hanks' Balanced Salt Solution
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Hydrocortisone Stock SolutionCell culture supplement
总览
EpiCult™-B Human Medium Kit 是一款无血清培养基，专为培养人乳腺腔细胞和肌上皮细胞而优化。该培养基非常适用于在乳腺集落形成单位（CFU）实验中，联合使用辐照处理的饲养层细胞（如 NIH 3T3）进行双能性、腔型限制性和肌上皮限制性乳腺上皮祖细胞的生长和功能评估。
本试剂盒包含 EpiCult™-B 基础培养基（人）和 EpiCult™-B 增殖添加剂（人）。需添加氢化可的松原液（产品号 #07925）。
本试剂盒包含 EpiCult™-B 基础培养基（人）和 EpiCult™-B 增殖添加剂（人）。需添加氢化可的松原液（产品号 #07925）。
本试剂盒包含 EpiCult™-B 基础培养基（人）和 EpiCult™-B 增殖添加剂（人）。需添加氢化可的松原液（产品号 #07925）。
本试剂盒包含 EpiCult™-B 基础培养基（人）和 EpiCult™-B 增殖添加剂（人）。需添加氢化可的松原液（产品号 #07925）。
亚型
专用培养基
细胞类型
乳腺细胞
种属
人
应用
细胞培养，克隆筛选
品牌
EpiCult
研究领域
上皮细胞研究
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (6)
文献 (9)
Molecular decoy to the Y-box binding protein-1 suppresses the growth of breast and prostate cancer cells whilst sparing normal cell viability. PloS one 2010 JAN
Abstract
The Y-box binding protein-1 (YB-1) is an oncogenic transcription/translation factor that is activated by phosphorylation at S102 whereby it induces the expression of growth promoting genes such as EGFR and HER-2. We recently illustrated by an in vitro kinase assay that a novel peptide to YB-1 was highly phosphorylated by the serine/threonine p90 S6 kinases RSK-1 and RSK-2, and to a lesser degree PKCα and AKT. Herein, we sought to develop this decoy cell permeable peptide (CPP) as a cancer therapeutic. This 9-mer was designed as an interference peptide that would prevent endogenous YB-1(S102) phosphorylation based on molecular docking. In cancer cells, the CPP blocked P-YB-1(S102) and down-regulated both HER-2 and EGFR transcript level and protein expression. Further, the CPP prevented YB-1 from binding to the EGFR promoter in a gel shift assay. Notably, the growth of breast (SUM149, MDA-MB-453, AU565) and prostate (PC3, LNCap) cancer cells was inhibited by ∼90% with the CPP. Further, treatment with this peptide enhanced sensitivity and overcame resistance to trastuzumab in cells expressing amplified HER-2. By contrast, the CPP had no inhibitory effect on the growth of normal immortalized breast epithelial (184htert) cells, primary breast epithelial cells, nor did it inhibit differentiation of hematopoietic progenitors. These data collectively suggest that the CPP is a novel approach to suppressing the growth of cancer cells while sparing normal cells and thereby establishes a proof-of-concept that blocking YB-1 activation is a new course of cancer therapeutics.
Association of reactive oxygen species levels and radioresistance in cancer stem cells. Nature 2009 APR
Abstract
The metabolism of oxygen, although central to life, produces reactive oxygen species (ROS) that have been implicated in processes as diverse as cancer, cardiovascular disease and ageing. It has recently been shown that central nervous system stem cells and haematopoietic stem cells and early progenitors contain lower levels of ROS than their more mature progeny, and that these differences are critical for maintaining stem cell function. We proposed that epithelial tissue stem cells and their cancer stem cell (CSC) counterparts may also share this property. Here we show that normal mammary epithelial stem cells contain lower concentrations of ROS than their more mature progeny cells. Notably, subsets of CSCs in some human and murine breast tumours contain lower ROS levels than corresponding non-tumorigenic cells (NTCs). Consistent with ROS being critical mediators of ionizing-radiation-induced cell killing, CSCs in these tumours develop less DNA damage and are preferentially spared after irradiation compared to NTCs. Lower ROS levels in CSCs are associated with increased expression of free radical scavenging systems. Pharmacological depletion of ROS scavengers in CSCs markedly decreases their clonogenicity and results in radiosensitization. These results indicate that, similar to normal tissue stem cells, subsets of CSCs in some tumours contain lower ROS levels and enhanced ROS defences compared to their non-tumorigenic progeny, which may contribute to tumour radioresistance.
Transcriptome analysis of the normal human mammary cell commitment and differentiation process. Cell stem cell 2008 JUL
Abstract
Mature mammary epithelial cells are generated from undifferentiated precursors through a hierarchical process, but the molecular mechanisms involved, particularly in the human mammary gland, are poorly understood. To address this issue, we isolated highly purified subpopulations of primitive bipotent and committed luminal progenitor cells as well as mature luminal and myoepithelial cells from normal human mammary tissue and compared their transcriptomes obtained using three different methods. Elements unique to each subset of mammary cells were identified, and changes that accompany their differentiation in vivo were shown to be recapitulated in vitro. These include a stage-specific change in NOTCH pathway gene expression during the commitment of bipotent progenitors to the luminal lineage. Functional studies further showed NOTCH3 signaling to be critical for this differentiation event to occur in vitro. Taken together, these findings provide an initial foundation for future delineation of mechanisms that perturb primitive human mammary cell growth and differentiation.
更多信息
| 种属 | Human |
|---|---|
| 配方类别 | Serum-Free |
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