EpiCult™ Plus培养基

用于培养人、小鼠和大鼠上皮干细胞

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产品号 #（选择产品）

产品号 #06070_C

用于培养人、小鼠和大鼠上皮干细胞

产品优势

这是一种无血清、无牛垂体提取物（BPE）的细胞培养基，与传统培养基相比，可显著促进上皮干细胞的增殖。
细胞可持续扩增超过 30 次群体倍增而不衰老
可维持具备分化潜能的上皮干细胞，分化为来源组织中的多种细胞类型
在不同传代和实验之间表现出高度一致性

产品组分包括

EpiCult™Plus基础培养基，490 mL
EpiCult™Plus添加物，10mL

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要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

Trypsin-EDTA (0.25%)
Enzymatic cell dissociation reagent
Hydrocortisone Stock Solution
Cell culture supplement
CellAdhereᵀᴹ Collagen I-Coated Plate
Sterile flat-bottom, tissue culture-treated, collagen I-coated multiwell plate with lid; 6-, 12-, 24-, 48-, 96-well formats
D-PBS (Without Ca++ and Mg++)
Dulbecco’s phosphate-buffered saline without calcium and magnesium
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

EpiCult™ Plus 培养基是一种无血清和无双酚A (BPE) 的培养基，用于扩增人、小鼠和大鼠乳腺、前列腺以及其他复层和假复层上皮组织的上皮干细胞。上皮细胞可在 EpiCult™ Plus 培养基中扩增多次传代，并可进行 30 次以上的群体倍增；对于某些人乳腺细胞，可能需要添加胎牛血清。

EpiCult™ Plus 培养基不建议用于培养人角质形成细胞或原代人呼吸道细胞；对于原代人呼吸道细胞，我们建议使用 PneumaCult™-Ex Plus 培养基（产品号 #05040）。
注意：配制完全 EpiCult™ Plus 培养基需要氢化可的松原液（产品号 #07925）。

亚型
专用培养基

细胞类型
上皮细胞

种属
人

应用
细胞培养

品牌
EpiCult

研究领域
上皮细胞研究

制剂类别
无血清

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实验数据

Figure 1. EpiCult™ Plus Enables the Long-Term Growth of Epithelial Stem Cells From Prostate and Mammary Tissues

(A) Mouse mammary, (B) mouse prostate, and (C) human prostate samples were established and expanded in EpiCult™ Plus. Growth curves from three independent samples are shown here.

Human Mammary Epithelial Cells Expand More Rapidly When Cultured in EpiCult™ Plus Compared to Conventional Epithelial Cell Expansion Media

Figure 2. Human Mammary Epithelial Cells Expand More Rapidly When Cultured in EpiCult™ Plus Compared to Conventional Epithelial Cell Expansion Media

Human mammary epithelial cells expanded in EpiCult™ Plus (solid line, solid markers) can be grown beyond 20 population doublings while cells grown in conventional epithelial cell expansion medium (dotted line, solid markers) senesced after approximately 10 population doublings. The addition of FBS to EpiCult™ Plus (solid line, open markers) allowed further expansion of the human mammary epithelial cells.

Figure 3. Cells Cultured in EpiCult™ Plus are Maintained in a Basal-Like State

EpiCult™ Plus maintains epithelial stem cells in a basal-like state as observed by the marker expression of CD49f in (A) mouse mammary, (B) mouse prostate, (C) human mammary, and (D) human prostate epithelial cells that were cultured in EpiCult™ Plus for a minimum of 8 passages. Flow cytometry analysis shows > 99% expression of CD49f across tissues.

Figure 4. Mouse Prostate and Mammary Epithelial Cells Expanded with EpiCult™ Plus Can Be Differentiated as Organoids

Mouse epithelial cells were expanded in EpiCult™ Plus and differentiated into either (A,B) prostate or (C,D) mammary organoids. (B,D) Both mouse prostate and mammary organoids express the luminal marker keratin 8 (green) and the basal marker keratin 14 (red) as shown by immunofluorescence microscopy; nuclei are counterstained with DAPI. This data demonstrates the compatibility of EpiCult™ Plus with both mouse prostate and mammary workflows that may require the robust upstream expansion of epithelial cells.

Figure 5. Human Prostate Epithelial Cells Expanded in EpiCult™ Plus Can Be Differentiated as Organoids

Freshly dissociated human prostate cells were (A) seeded and expanded in EpiCult™ Plus. After 5 days in culture, the cells were harvested and seeded into human prostate organoid medium and maintained for an additional (B) 11 days. (C) The resulting organoids contain a cell-dense core composed of luminal cells expressing keratin 8 (green) and androgen receptors (magenta), while basal cells expressing keratin 14 (red) line the outer side of the prostate organoid. This demonstrates that EpiCult™ Plus supports the robust expansion of human prostate organoid-forming cells.