StemSpan™ serum-free expansion media is recommended for the expansion and culture of hematopoietic cells, when supplemented with appropriate cytokines and supplements. We offer a number of StemSpan™ Cytokine Cocktails (CC) and Supplements optimized for the expansion of human CD34+ progenitors cells in serum-free culture. To help you select the appropriate StemSpan™ cytokine supplement for your application, we’ve outlined some of the features and differences between these products below:

PRODUCT	CATALOG # (SIZE)	COMPONENTS	FEATURES	PERFORMANCE* (in 7 day cultures)
StemSpan™ CC100	02690 (1 mL, 100X)	rh Flt3L rh SCF rh IL-3 rh IL-6	Contains both early- and late-acting cytokines Stimulates rapid and strong proliferation of human hematopoietic cells, including CD34+ progenitors Generates large numbers of hematopoietic cells in culture, including CD34+ cells and more mature cells. Preferable over CC110 if high cell yields are important	TNC fold expansion: ~20-60-fold CD34+ cells fold expansion: at most ~10-fold
StemSpan™ CC110	02697 (1 mL, 100X)	rh Flt3L rh SCF rh TPO	Contains early-acting cytokines Stimulates proliferation of human hematopoietic cells, including CD34+ progenitors Generates similar expansion of CD34+ cells, but lower total nucleated cell (TNC) yields and higher CD34+ cell frequencies than CC100 Preferable over CC100 if high CD34+ cell purity is important Used primarily to activate stem and immature progenitor cell cycling in short term cultures (i.e. for lentiviral or retroviral gene transfer procedures aimed at genetic modification of stem/progenitor cells).
StemSpan™ CD34+ Expansion Supplement	02691 (10 mL, 10X)	rh Flt3L rh SCF rh IL-3 rh IL-6 rh TPO other additives	Stimulates selective expansion of human CD34+ hematopoietic stem and progenitor cells (HSPCs) Generates similar overall expansion of TNCs, but much higher expansion of CD34+ cells compared to CC100 and CC110 Preferable over CC100 and CC110 if high CD34+ cell yields and purity are important. Used to achieve high cell yields and also maintain high CD34 expression in cultures.	TNC fold expansion: ~20-60-fold CD34+ cells fold expansion: >10 to 40-fold

*NOTE: The magnitude of expansion of hematopoietic cells and progenitors is variable between donors and is also dependent on a number of factors including: the cell source, input cell population, culture medium, cell density, time in culture and refeeding or passaging procedures.

These StemSpan™ Cytokine Cocktails and Supplements are intended for use in combination with our StemSpan™ serum-free expansion media, including StemSpan™ SFEM, SFEM II and ACF.

For further information on our StemSpan™ products, please contact techsupport@stemcell.com.