若您需要咨询产品或有任何技术问题,请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系

加替沙星(Gatifloxacin)

抗生素;抑制细菌DNA旋转酶(gyrase)和拓扑异构酶IV(topoisomerase IV)

产品号 #(选择产品)

产品号 #72752_C

抗生素;抑制细菌DNA旋转酶(gyrase)和拓扑异构酶IV(topoisomerase IV)

总览

加替沙星是一种氟喹诺酮类抗生素,抑制细菌DNA旋转酶(IC50= 0.109µg/ml)和拓扑异构酶IV (IC50= 13.8µg/ml)(Takei et al.)。对HeLa细胞拓扑异构酶II 的抑制效果较弱(IC50= 265µg/ml)(Takei et al.)。

维持和自我更新
·促进培养的人和小鼠胚胎干细胞的自我更新(Desbordes et al.)。

细胞类型
多能干细胞
 
种属
人,小鼠,非人灵长类,其它细胞系,大鼠
 
应用
抗生素,扩增,培养
 
研究领域
干细胞生物学
 
CAS 编号
112811-59-3
 
化学式
C₁₉H₂₂FN₃O₄
 
纯度
≥98%
 

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
Gatifloxacin
Catalog #
72752
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Gatifloxacin
Catalog #
72752
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (1)

文献 (2)

High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Desbordes SC et al. Cell stem cell 2008 JUN

Abstract

High-throughput screening (HTS) of chemical libraries has become a critical tool in basic biology and drug discovery. However, its implementation and the adaptation of high-content assays to human embryonic stem cells (hESCs) have been hampered by multiple technical challenges. Here we present a strategy to adapt hESCs to HTS conditions, resulting in an assay suitable for the discovery of small molecules that drive hESC self-renewal or differentiation. Use of this new assay has led to the identification of several marketed drugs and natural compounds promoting short-term hESC maintenance and compounds directing early lineage choice during differentiation. Global gene expression analysis upon drug treatment defines known and novel pathways correlated to hESC self-renewal and differentiation. Our results demonstrate feasibility of hESC-based HTS and enhance the repertoire of chemical compounds for manipulating hESC fate. The availability of high-content assays should accelerate progress in basic and translational hESC biology.
Inhibitory activities of gatifloxacin (AM-1155), a newly developed fluoroquinolone, against bacterial and mammalian type II topoisomerases. Takei M et al. Antimicrobial agents and chemotherapy 1998 OCT

Abstract

We determined the inhibitory activities of gatifloxacin against Staphylococcus aureus topoisomerase IV, Escherichia coli DNA gyrase, and HeLa cell topoisomerase II and compared them with those of several quinolones. The inhibitory activities of quinolones against these type II topoisomerases significantly correlated with their antibacterial activities or cytotoxicities (correlation coefficient [r] = 0.926 for S. aureus, r = 0.972 for E. coli, and r = 0.648 for HeLa cells). Gatifloxacin possessed potent inhibitory activities against bacterial type II topoisomerases (50% inhibitory concentration [IC50] = 13.8 microg/ml for S. aureus topoisomerase IV; IC50 = 0.109 microg/ml for E. coli DNA gyrase) but the lowest activity against HeLa cell topoisomerase II (IC50 = 265 microg/ml) among the quinolones tested. There was also a significant correlation between the inhibitory activities of quinolones against S. aureus topoisomerase IV and those against E. coli DNA gyrase (r = 0.969). However, the inhibitory activity against HeLa cell topoisomerase II did not correlate with that against either bacterial enzyme. The IC50 of gatifloxacin for HeLa cell topoisomerase II was 19 and was more than 2,400 times higher than that for S. aureus topoisomerase IV and that for E. coli DNA gyrase. These ratios were higher than those for other quinolones, indicating that gatifloxacin possesses a higher selectivity for bacterial type II topoisomerases.

更多信息

更多信息
种属 Human, Mouse, Non-Human Primate, Other, Rat
Cas Number 112811-59-3
Chemical Formula C₁₉H₂₂FN₃O₄
纯度 ≥ 98%
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息,请访问 STEMCELL.CN/COMPLIANCE.
Copyright © 2025 by STEMCELL Technologies. All rights reserved.