TeSR™-E7™ is a xeno-free and defined reprogramming culture medium optimized for the generation of human induced pluripotent stem (iPS) cells without the use of feeders. This video describes the characteristics of reprogrammed iPS cell colonies and provides instructions for manually harvesting putative iPS cell colonies for replating and further expansion. View additional technical tips on reprogramming somatic cells using TeSR™-E7™ reprogramming culture medium.