EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #06030_C
用于建立和维持小鼠肝祖类器官的培养基
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
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Anti-Adherence Rinsing SolutionRinsing solution for cultureware to prevent cell adhesion
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Collagenase Type IV (1 mg/mL)Cell dissociation reagent
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Dispase (1 U/mL)1 U/mL dispase in DMEM/F-12
总览
HepatiCult™ 类器官生长培养基 (小鼠)是一款无血清的成分明确的培养基，用于小鼠肝脏祖细胞类器官的建立和维持。这些类器官或称“迷你肝脏”，提供了一个体外的器官型培养系统，用于研究肝脏干细胞和祖细胞。在HepatiCult™培养基中生长的类器官具有表达标记肝脏干细胞和祖细胞（PROM1、AXIN2、SOX9和CD44）、导管细胞（KRT19和HNF1b）以及肝细胞（HNF4a、AFP）基因的上皮。肝类器官可以每4 - 7天传代一次，可进行冷冻保存，也可进行下游分化。
HepatiCult™支持将小鼠肝类器官在Corning® Matrigel®的胶滴中，或在稀释的Matrigel®悬液中进行培养。类器官培养提供了一种便捷的体外方法，可在生理相关的体系中对肝脏上皮进行表征，同时减少对动物的使用。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB联系，以获取商业用途许可或HUB许可相关的说明。
HepatiCult™支持将小鼠肝类器官在Corning® Matrigel®的胶滴中，或在稀释的Matrigel®悬液中进行培养。类器官培养提供了一种便捷的体外方法，可在生理相关的体系中对肝脏上皮进行表征，同时减少对动物的使用。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB联系，以获取商业用途许可或HUB许可相关的说明。
HepatiCult™支持将小鼠肝类器官在Corning® Matrigel®的胶滴中，或在稀释的Matrigel®悬液中进行培养。类器官培养提供了一种便捷的体外方法，可在生理相关的体系中对肝脏上皮进行表征，同时减少对动物的使用。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB联系，以获取商业用途许可或HUB许可相关的说明。
HepatiCult™支持将小鼠肝类器官在Corning® Matrigel®的胶滴中，或在稀释的Matrigel®悬液中进行培养。类器官培养提供了一种便捷的体外方法，可在生理相关的体系中对肝脏上皮进行表征，同时减少对动物的使用。
如果您打算将本产品用于商业目的，请通过www.huborganoids.nl与HUB联系，以获取商业用途许可或HUB许可相关的说明。
亚型
专用培养基
细胞类型
肝细胞
种属
小鼠
应用
细胞培养，扩增，培养，类器官培养
品牌
HepatiCult
研究领域
癌症，疾病建模，药物发现和毒理检测，上皮细胞研究，干细胞生物学
制剂类别
无血清
实验数据
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (20)
文献 (2)
The CellRaft AIR? system: A novel system enabling organoid imaging, identification, and isolation SLAS Discovery 2022 4
Abstract
Three-dimensional (3D) culture systems have been developed that can re-capitulate organ level responses, simulate compound diffusion through complex structures, and assess cellular heterogeneity of tissues, making them attractive models for advanced in vitro research and discovery. Organoids are a unique subtype of 3D cell culture that are grown from stem cells, are self-organizing, and closely replicate in vivo pathophysiology. Organoids have been used to understand tissue development, model diseases, test drug sensitivity and toxicity, and advance regenerative medicine. However, traditional organoid culture methods are inadequate because they are low throughput and ill-suited for single organoid imaging, phenotypic assessment, and isolation from heterogenous organoid populations. To address these bottlenecks, we have adapted our tissue culture consumable and instrumentation to enable automated imaging, identification, and isolation of individual organoids. Organoids grown on the 3D CytoSort? Array can be reliably tracked, imaged, and phenotypically analyzed using brightfield and fluorescent microscopy as they grow over time, then released and transferred fully intact for use in downstream applications. Using mouse hepatic and pancreatic organoids, we have demonstrated the use of this technology for single-organoid imaging, clonal organoid generation, parent organoid subcloning, and single-organoid RNA extraction for downstream gene expression or transcriptomic analysis. The results validate the ability of the CellRaft AIR? System to facilitate efficient, user-friendly, and automated workflows broadly applicable to organoid research by overcoming several pain points: 1) single organoid time-course imaging and phenotypic assessment, 2) establishment of single cell-derived organoids, and 3) isolation and retrieval of single organoids for downstream applications.
Modulation of the extrinsic cell death signaling pathway by viral Flip induces acute-death mediated liver failure. Cell death {\&} disease 2019
Abstract
During viral infections viruses express molecules that interfere with the host-cell death machinery and thus inhibit cell death responses. For example the viral FLIP (vFLIP) encoded by Kaposi's sarcoma-associated herpesvirus interacts and inhibits the central cell death effector, Caspase-8. In order to analyze the impact of anti-apoptotic viral proteins, like vFlip, on liver physiology in vivo, mice expressing vFlip constitutively in hepatocytes (vFlipAlbCre+) were generated. Transgenic expression of vFlip caused severe liver tissue injury accompanied by massive hepatocellular necrosis and inflammation that finally culminated in early postnatal death of mice. On a molecular level, hepatocellular death was mediated by RIPK1-MLKL necroptosis driven by an autocrine TNF production. The loss of hepatocytes was accompanied by impaired bile acid production and disruption of the bile duct structure with impact on the liver-gut axis. Notably, embryonic development and tissue homeostasis were unaffected by vFlip expression. In summary our data uncovered that transgenic expression of vFlip can cause severe liver injury in mice, culminating in multiple organ insufficiency and death. These results demonstrate that viral cell death regulatory molecules exhibit different facets of activities beyond the inhibition of cell death that may merit more sophisticated in vitro and in vivo analysis.
更多信息
| 种属 | Mouse |
|---|---|
| 配方类别 | Serum-Free |
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法律声明:
This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. 质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
