Hoechst 33342（盐酸盐）

用于标记和观察细胞核的荧光染料

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产品号 #100-1540_C

用于标记和观察细胞核的荧光染料

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总览

Hoechst 33342（盐酸盐）是Hoechst荧光染料系列的一员，该系列还包括Hoechst 33258 和 Hoechst 34580。Hoechst 33342（盐酸盐）是一种可透过细胞膜的荧光染料，适用于对活细胞或固定细胞的细胞核进行染色和可视化观察。Hoechst 33342（盐酸盐）的激发波长约为 350 nm，最大发射波长为 461 nm，发出蓝色/青色荧光。与其它Hoechst染料相比，Hoechst 33342 由于含有一个额外的疏水性乙基基团，具备更强的细胞通透性（Bucevičius et al.）。在活细胞染色实验中，建议使用 Hoechst 33342（盐酸盐）替代 DAPI，因为其毒性更低，对细胞活性影响较小，更有利于维持染色后细胞的生理状态。此外，Hoechst 33342（盐酸盐）也可作为固定样本的细胞核复染剂。

实验数据

Fluorescent Microscope Image of Cell Cultures

Figure 1. Hoechst 33342 Labels DNA in Live Cell Cultures

(A) Live HeLa cells were stained with 5 µM Hoechst 33342 (Hydrochloride) and then imaged using a fluorescence microscope. Image was taken at 200X magnification and is a cropped field of view. The image shows bright staining of several HeLa cell nuclei (blue). (B) Chemical structure of Hoechst 33342 (Hydrochloride).

Fluorescent Microscope Image of Cell Cultures Pre-Fixation and Post-Fixation

Figure 2. Hoechst 33342 (Hydrochloride) Brightly Labels DNA in Cell Cultures Pre-Fixation and Post-Fixation

Live HeLa cells were stained with (A) 5 µM or (B) 10 µM Hoechst 33342 (Hydrochloride; blue) for 30 minutes at 37°C, then imaged using a fluorescence microscope. (C, D) Images were taken at 200X magnification and are cropped field of views. The images show bright staining of DNA is maintained even after fixation with 4% formaldehyde.

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Hoechst 33342 (Hydrochloride)

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