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Hoechst 33342(盐酸盐)

用于标记和观察细胞核的荧光染料

产品号 #(选择产品)

产品号 #100-1540_C

用于标记和观察细胞核的荧光染料

总览

Hoechst 33342(盐酸盐)是Hoechst荧光染料系列的一员,该系列还包括Hoechst 33258 和 Hoechst 34580。Hoechst 33342(盐酸盐)是一种可透过细胞膜的荧光染料,适用于对活细胞或固定细胞的细胞核进行染色和可视化观察。Hoechst 33342(盐酸盐)的激发波长约为 350 nm,最大发射波长为 461 nm,发出蓝色/青色荧光。与其它Hoechst染料相比,Hoechst 33342 由于含有一个额外的疏水性乙基基团,具备更强的细胞通透性(Bucevičius et al.)。在活细胞染色实验中,建议使用 Hoechst 33342(盐酸盐)替代 DAPI,因为其毒性更低,对细胞活性影响较小,更有利于维持染色后细胞的生理状态。此外,Hoechst 33342(盐酸盐)也可作为固定样本的细胞核复染剂。

别名
双苯并咪唑
 
应用
鉴定
 
CAS 编号
875756-97-1
 
化学式
C27H28N6O· 3HCl
 
分子量
561.93 克/摩尔
 

实验数据

Fluorescent Microscope Image of Cell Cultures

Figure 1. Hoechst 33342 Labels DNA in Live Cell Cultures

(A) Live HeLa cells were stained with 5 µM Hoechst 33342 (Hydrochloride) and then imaged using a fluorescence microscope. Image was taken at 200X magnification and is a cropped field of view. The image shows bright staining of several HeLa cell nuclei (blue). (B) Chemical structure of Hoechst 33342 (Hydrochloride).

Fluorescent Microscope Image of Cell Cultures Pre-Fixation and Post-Fixation

Figure 2. Hoechst 33342 (Hydrochloride) Brightly Labels DNA in Cell Cultures Pre-Fixation and Post-Fixation

Live HeLa cells were stained with (A) 5 µM or (B) 10 µM Hoechst 33342 (Hydrochloride; blue) for 30 minutes at 37°C, then imaged using a fluorescence microscope. (C, D) Images were taken at 200X magnification and are cropped field of views. The images show bright staining of DNA is maintained even after fixation with 4% formaldehyde.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1540
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1540
Lot #
All
Language
English

更多信息

更多信息
Molecular Weight 561.93 g/mol
Alternative Names Bisbenzimide
Cas Number 875756-97-1
Chemical Formula C27H28N6O· 3HCl
质量保证:

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