In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media such as mTeSR™1 into mTeSR™ Plus. This video will cover the generation of cell aggregates and passaging protocol. 0:00 - Intro 1:23 - ReLeSR™ Passaging 3:14 - GCDR Passaging 5:56 - Plate Setup for mTeSR Plus Transition Watch the other videos in the series below: Video 1: Plate Coating Video 3: Maintenance and Morphological Assessment Learn more about mTeSR™ Plus hPSC maintenance media >

See a written version of this protocol >