In this series of three technical videos, we will guide you through the protocol for transitioning human pluripotent stem cells (hPSCs) cultured in feeder-free media, such as mTeSR™1 into mTeSR™ Plus. This video will provide step by step instructions for plate coating. 0:00 - Intro 0:29 - Matrigel Plate Coating 2:42 - Vitronectin XF™ Plate Coating Watch the other videos in the series below: Video 2: Generation of Cell Aggregates/Passaging Video 3: Maintenance and Morphological Assessment Learn more about mTeSR™ Plus hPSC maintenance media > View a written protocol on how to transition hPSCs from feeder-free media to mTeSR™ Plus.