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人C-反应蛋白 ELISA试剂盒

用于检测和测定人C-反应蛋白

产品号 #(选择产品)

产品号 #100-1144_C

用于检测和测定人C-反应蛋白

产品组分包括

  • 人C-反应蛋白ELISA试剂盒
    • 人C-反应蛋白ELISA酶标板,1x
    • 人c -反应蛋白HRP检测抗体,1瓶
    • 人C-反应蛋白标准品,1瓶
    • 洗涤缓冲液(20X), 30mL
    • 稀释缓冲液(20X), 12.5 mL
    • 过氧化物溶液,12.5 mL
    • TMB底物,12.5 mL
    • 终止液,11mL

总览

人C反应蛋白 (CRP) ELISA试剂盒用于定量检测血清中的CRP。CRP主要在肝脏中合成,响应促炎细胞因子,例如白细胞介素1 (IL-1)、白细胞介素6 (IL-6)和肿瘤坏死因子 (TNF)。CRP在识别和清除病原微生物、细胞碎片和凋亡细胞方面发挥关键作用。它与细菌上的磷酸胆碱以及染色质、组蛋白和snRNP等多种核成分结合。这激活补体系统并增强巨噬细胞的吞噬功能。在类风湿性关节炎、心血管疾病和感染等炎症状态下,CRP的表达会升高。它也可用作炎症的临床标志物,用于预测无症状个体的心血管疾病。

该检测基于夹心ELISA方法,将样品加入预先包被有针对人CRP特异性捕获抗体的ELISA可拆酶标板。通过添加辣根过氧化物酶 (HRP)偶联的检测抗体来检测捕获的CRP。添加显色酶底物3,3',5,5'-四甲基联苯胺 (TMB)后,产生显色产物,其强度与样品中人CRP的浓度成正比。

亚型
完整试剂盒
 
细胞类型
肝细胞,淋巴细胞,巨噬细胞,T 细胞
 
种属

 
应用
ELISAs
 
研究领域
免疫,感染性疾病(传染病)
 

实验数据

Representative Standard Curve Of Human C-Reactive Protein

Figure 1. Representative Standard Curve Of Human C-Reactive Protein

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 54.69 - 3500 pg/mL. The lower limit of detection (LLD) of this assay is 21.78 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 99 - 104% was determined by spiking defined amounts of analyte standard into serum samples in repeated experiments. The intra-assay precision of this assay is 7.1% (CV). The Inter-assay precision of this assay is 5.9% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 6
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Safety Data Sheet 7
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Safety Data Sheet 8
Catalog #
100-1144
Lot #
All
Language
English

更多信息

更多信息
种属 Human
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。
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