Human granulocyte-macrophage colony-stimulating factor (GM-CSF) ELISpot Kit is designed for the quantitative detection and enumeration of cells secreting human GM-CSF in a suspension of peripheral blood mononuclear cells (PBMCs). ELISpot is commonly used for evaluating specific immune responses, such as determining the frequency of activated T cell subsets in response to antigenic stimulation. GM-CSF is secreted by many cell types including the T helper (Th) subsets Th1, Th2, Th17, and ThGM cells as well as CD1a-reactive T cells involved in inflammatory and allergic diseases. GM-CSF can also be produced by monocytes and dendritic cells in response to pathogen recognition. ELISpot is highly sensitive and can detect secreted analytes at a single cell level. This assay is used in research of infectious diseases, cancers, allergies, and autoimmune diseases.
The assay is based on a modified version of the sandwich ELISA method, in which cells are added to ELISpot plates pre-coated with capture antibodies specific for the cytokine. The cytokine is then captured throughout the culture period and is detected by the addition of a biotinylated detection antibody, followed by streptavidin-alkaline phosphatase (SA-ALP), which binds to the biotinylated antibody. Addition of the chromogenic enzyme substrate 5-bromo-4-chloro-3-indolyl-1-phosphate (BCIP)/nitroblue tetrazolium (NBT), results in the formation of colored spots. The number, size, and intensity of these spots are directly proportional to the frequency of cytokine-secreting cells in the sample and the amount of analyte secreted.
Figure 1. Human GM-CSF ELISpot Kit Detects GM-CSF-Secreting Cells Following Immunogenic Stimulation.
ELISpot analysis of human peripheral blood mononuclear cells cultured in the absence of stimuli (negative control) or presence of lipopolysaccharide (LPS). Cells were seeded at 1 x 10^4 cells/well on Human GM-CSF pre-coated white plate and incubated at 37°C, 5% CO2 for 20 hours. Following incubation, ELISpot plates were processed as per the instructions on the Product Information Sheet. Wells were imaged and counted using an ELISpot reader.
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they
can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
EasySep™小鼠TIL（CD45）正选试剂盒
沪公网安备31010102008431号