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人IgE ELISA Kit

用于人免疫球蛋白E的检测和测定

产品号 #(选择产品)

产品号 #02032_C

用于人免疫球蛋白E的检测和测定

产品组分包括

  • 人IgE酶联免疫吸附测定试剂盒,2板(目录#02032)
    • 人IgE酶联免疫吸附试验板,2x
    • 人IgE标准品,1瓶
    • Human IgE Biotinylated Detection Antibody, 30µL
    • Sa-hrp, 30µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20X), 120 mL
    • ELISA稀释液,120 mL
    • SA-HRP稀释液,25ml
    • TMB底物,25ml
    • 停止液,25ml
    • 粘接板盖,6个
  • 人IgE酶联免疫吸附测定试剂盒,10板(目录#02033)
    • 人IgE ELISA板,10x
    • 人IgE标准品,1瓶
    • Human IgE Biotinylated Detection Antibody, 130µL
    • Sa-hrp, 130µl
    • 标准重组缓冲液B, 1ml
    • 洗涤缓冲液(20倍),5 × 120毫升
    • ELISA稀释剂,3 × 120ml
    • SA-HRP稀释剂,120 mL
    • TMB底物,120 mL
    • 停止液,120ml
    • 胶粘剂板盖,30x

总览

人免疫球蛋白E (IgE)酶联免疫吸附测定试剂盒是专为定量检测和测量人的IgE在生物流体,如血清,血浆和细胞培养上清。IgE主要存在于肺、皮肤和粘膜中,是血清中最不常见的免疫球蛋白。IgE通过与Fcε受体I (Fcε ri)相互作用,刺激嗜酸性粒细胞、肥大细胞和嗜碱性粒细胞的脱颗粒,在过敏反应中发挥重要作用。

该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的免疫球蛋白特异性抗体。捕获的免疫球蛋白通过添加生物素化检测抗体检测,然后添加链亲和素-辣根过氧化物酶(SA-HRP),它与生物素化抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中免疫球蛋白的浓度成正比。免疫球蛋白的浓度是通过与平行分析的免疫球蛋白标准品的连续稀释度进行比较来确定的。

Subtype
Complete Kits
 
Cell Type
B Cells, Hybridomas
 
Species
Human
 
Area of Interest
Hybridoma Generation, Immunology
 

实验数据

Representative Standard Curve

• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.069 ng/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.
• Recovery: A mid-curve recovery of 80 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.8% (CV). The inter-assay precision of this assay is 5.5% (CV).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Catalog #
02033, 02032
Lot #
All
Language
English
Catalog #
02033, 02032
Lot #
All
Language
English
Catalog #
02033, 02032
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计,适用于工作流程中的高亮阶段。探索这些工作流程,了解更多我们为各研究领域提供的其他配套产品。

相关材料与文献

技术资料 (2)

更多信息

更多信息
种属 Human
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