产品号 #02012_C
用于人白细胞介素10的检测和测定
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用于人白细胞介素10的检测和测定
人白细胞介素10 (IL-10)酶联免疫吸附测定试剂盒是专为定量检测和测量人白细胞介素10在生物液体,如血清,血浆和细胞培养上清。IL-10主要由树突状细胞、巨噬细胞和CD4+ T细胞产生。IL-10对调节性T细胞的功能很重要,它可以促进B细胞增殖、免疫球蛋白分泌和MHC II类表达。
该检测基于夹心ELISA方法,将样品添加到酶联免疫吸附试验(ELISA)带材板上,该带材板上预先涂有捕获的细胞因子特异性抗体。捕获的细胞因子通过添加生物素化的检测抗体来检测,然后添加链霉亲和素-辣根过氧化物酶,该酶与生物素化的抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺(TMB)可得到着色产物,其强度与样品中细胞因子的浓度成正比。细胞因子的浓度是通过与平行分析的细胞因子标准品的连续稀释进行比较来确定的。
Subtype
Complete Kits
Cell Type
Dendritic Cells, Macrophages, Monocytes, T Cells, CD4+
Species
Human
Application
ELISA
Area of Interest
Immunology
Representative Standard Curve
• Reportable range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.5 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC* international standard 93/722.
• Recovery: A mid-curve recovery of 76 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.5% (CV). The inter-assay precision of this assay is 9.8% (CV).
*National Institute of Biological Standards and Control, Potters Bar, Hertfordshire EN6 3QG, UK.
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种属 | Human |
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人T细胞激活扩增试剂
用于T细胞扩增的无血清和无异种成分培养基
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