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人IL-6ELISA试剂盒

用于人白细胞介素-6的检测和测定

产品号 #(选择产品)

产品号 #100-1138_C

用于人白细胞介素-6的检测和测定

产品组分包括

  • 人IL-6ELISA试剂盒
    • 人IL-6ELISA酶标板
    • 人IL-6 HRP检测抗体,1瓶
    • 人IL-6标准品,1瓶
    • 洗涤缓冲液(20X), 30ml
    • 稀释缓冲液(20X), 12.5 mL
    • 过氧化物溶液,12.5 mL
    • TMB底物,12.5 mL
    • 终止液,11mL

总览

人白细胞介素 6 (IL-6) ELISA 试剂盒用于定量检测和测定血清和细胞培养上清液等生物体液中的 IL-6。IL-6 主要由巨噬细胞、成纤维细胞、活化 T 细胞和内皮细胞产生。IL-6 通过 IL-6R 受体进行信号传导,激活糖蛋白 130 并随后进行下游信号传导。它是浆细胞瘤和骨髓瘤的重要生长因子,并能增强 B 细胞增殖。IL-6 还参与造血、骨代谢和癌症进展,并被定义为指导从先天免疫向获得性免疫转变的重要细胞因子。

该检测基于夹心 ELISA 方法,将样品加入预先包被有针对人 IL-6 的特异性捕获抗体的 ELISA可拆酶标板。通过添加辣根过氧化物酶 (HRP) 偶联的检测抗体来检测捕获的 IL-6。添加显色酶底物 3,3'5,5' - 四甲基联苯胺 (TMB) 会产生有色产物,其强度与样品中人 IL-6 的浓度成正比。

亚型
完整试剂盒
 
细胞类型
内皮细胞,成纤维细胞,巨噬细胞,T 细胞
 
种属

 
应用
ELISAs
 
研究领域
免疫,感染性疾病(传染病)
 

实验数据

Representative Standard Curve Of Human IL-6

Figure 1. Representative Standard Curve Of Human IL-6

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 2.81 - 180 pg/mL. The lower limit of detection (LLD) of this assay is 0.58 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 97 - 117% or 72 - 78% was determined by spiking defined amounts of analyte standard into cell culture supernatant or serum samples, respectively. The intra-assay precision of this assay is 3.9% (CV). The Inter-assay precision of this assay is 6.4% (CV)

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明,或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1138
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1138
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All
Language
English
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Safety Data Sheet 2
Catalog #
100-1138
Lot #
All
Language
English
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Safety Data Sheet 3
Catalog #
100-1138
Lot #
All
Language
English
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Safety Data Sheet 4
Catalog #
100-1138
Lot #
All
Language
English
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Safety Data Sheet 5
Catalog #
100-1138
Lot #
All
Language
English
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Safety Data Sheet 6
Catalog #
100-1138
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Catalog #
100-1138
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Catalog #
100-1138
Lot #
All
Language
English

更多信息

更多信息
种属 Human
质量保证:

产品仅供研究使用,不用于针对人或动物的诊断或治疗。
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