EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-1794_C
卵泡抑素抗性激活素 A，无动物成分
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总览
卵泡抑素抗性激活素 A（FRACTA）是经过改造的Activin A 变体，其设计的目的是减少与 Activin A 天然抑制因子 Follistatin 的结合能力。Activin A是转化生长因子β (TGF-β)家族的成员，由多种细胞在发育过程中产生（Gurdon et al.）。其为一种二硫连接的同型二聚体（两条β - A链组成），与I型（Act RI-A和Act RI-B）和II型（Act RII-A和Act RII-B）丝氨酸-苏氨酸激酶受体复合物结合（Attisano et al.）。Activin主要通过SMAD2/3信号通路发挥作用，调控细胞增殖、分化、伤口愈合、凋亡和代谢（McDowell et al.）。Follistatin 通过阻断 Activin A 与Ⅱ型受体的结合位点，从而抑制 Activin A 信号传导（Harrington et al.）。Activin A可维持人胚胎干细胞的未分化状态(James et al.; Xiao et al.)，也促进人胚胎干细胞向定形内胚层的分化（D’Amour et al.）。本产品为无动物成分（ACF）级别。
亚型
细胞因子
别名
Activin beta-A 链；EDF；红细胞分化蛋白；FRACTA；FRP；FSH 释放蛋白；INHBA；抑制素 betaA 链；抑制素 beta-1
细胞类型
内胚层，PSC衍生，中胚层，PSC衍生，其它细胞系，多能干细胞
种属
人，小鼠，大鼠
研究领域
上皮细胞研究，干细胞生物学
制剂类别
Animal Component-Free
纯度
SDS-PAGE 检测：≥ 98%
实验数据
Figure 1. Biological Activity and Molecular Mass of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF
(A) The biological activity of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF was tested using an activin-responsive luciferase reporter assay in HEK293T cells. Firefly luciferase activity is normalized to control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which activin response is at 50% of maximum. The EC50 in the above example is 8.79 pM (0.23 ng/mL).
(B) 1 μg of Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human/Mouse/Rat Recombinant Follistatin-Resistant Activin A, ACF has a predicted molecular mass of 26 kDa (dimer).
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Product Name
Catalog #
Lot #
Language
应用领域
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Research Area
Workflow Stages
Workflow Stages for Mouse Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
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更多信息
| 种属 | Human, Mouse, Rat |
|---|---|
| 配方类别 | Animal Component-Free |
| Alternative Names | Activin beta-A chain, EDF, Erythroid differentiation protein, FRACTA, FRP, FSH-releasing protein, INHBA, Inhibin betaA chain, Inhibin beta-1 |
| 纯度 | ≥ 98% by SDS-PAGE |
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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