冻存的人外周血白细胞单采术样本

冻存的人原代细胞

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产品号 #200-0130_C

冻存的人原代细胞

产品优势

可从单个供体中分离出大量的单个核细胞。

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New data and packaging, same high quality! Flow cytometry plots are now included on the Certificate of Analysis (CoA) for frozen leukopaks manufactured after May 2022. See Figure 1 in the data section below to view representative plots. Your leukopak may come with external packaging to ensure you get the highest quality product possible upon delivery. Remove all external packaging for optimal thawing performance. Request the CoA for your frozen leukopaks to see the data, and browse our Frequently Asked Questions (FAQs) on Primary Cells for more information.

总览

实验数据

产品说明书及文档

应用领域

总览

可为您提供大量的白细胞，包括外周血单个核细胞（PBMCs）以开始实验。作为一种冻存产品，该冻存的白细胞单采术样本提供来自特定供体的样本，可在用户准备就绪时进行处理。我们提供个性化的服务、定制产品、灵活的交付周期，以确保您获得所需的细胞。

每个冻存的白细胞单采术样本均采用机构审查委员会 (IRB) 批准的知情同意书和方案，从单个正常供体体内采集高浓度、小体积的单采血液。每个完整规格的白细胞单采术样本均使用Spectra Optia®Apheresis系统，采用酸性柠檬酸葡萄糖溶液A （ACDA）作为抗凝剂，以约2-3倍血液体积制成。采集的样本随后在控温冰箱中冻存。我们可根据需求提供高分辨率HLA分型（I类和II类等位基因以及CMV状态）。选择产品选项后，您可以在上方的评论框中填写供体信息（例如BMI范围、吸烟状况、种族等）。供体需接受HIV-1、HIV-2、乙肝和丙肝筛查。

某些产品仅在特定地区出售。请通过info.cn@stemcell.com联系您当地的销售代表或技术支持以获取更多信息。

欲了解更多信息，请浏览有关原代细胞的常见问题解答（FAQs）。

亚型
冻存

细胞类型
白细胞单采样本，单个核细胞

种属
人

细胞和组织来源
外周血

供体状态
Normal

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实验数据

Figure 1. Typical Flow Cytometric Analysis Profile of a Leukopak Before Cryopreservation

Representative gating strategy for flow cytometric immunophenotyping of the different cell subpopulations present in a leukopak product prior to cryopreservation. The CD45 plot was gated on viable single cells while all other plots were gated on viable CD45+ single cells. In the above example, the cell frequencies (as a percentage of the overall leukopak product) are as follows: monocytes (CD14+) 19.9%, B cells (CD19+) 13.9%, NK cells (CD3-CD56+) 6.3%, Helper T cells (CD3+CD4+) 28.5%, and Cytotoxic T cells (CD3+CD8+) 13.5%.

Figure 2. Mean Percentages of Cell Subpopulations in a Leukopak

Representative chart showing the average frequencies of major immune subsets in leukopak products, as measured by flow cytometry prior to cryopreservation. Values shown are mean percentages of total viable leukocytes present in a leukopak (n ≥ 30).