重组人EPO （CHO-表达）

促红细胞生成素

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产品号 #（选择产品）

产品号 #78007_C

促红细胞生成素

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总览

实验数据

产品说明书及文档

应用领域

总览

促红细胞生成素（EPO）是红细胞生成的主要生理调节因子，是一种糖蛋白类生长因子，主要在肾脏中因低氧或贫血状态而产生。EPO通过结合同二聚体细胞表面受体来促进红细胞生成，激活JAK2/STAT5、PI3K/AKT和MAPK通路，刺激红细胞祖细胞的增殖和分化(Jelkmann，《输血医学与血液疗法》，2013；Kuhrt & Wojchowski《血液》，2015)。

亚型
细胞因子

细胞类型
造血干/祖细胞，中胚层，PSC衍生，多能干细胞

种属
人

研究领域
干细胞生物学

纯度
≥ 95 %

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实验数据

(A) The biological activity of Human Recombinant EPO was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which the cell proliferation is at 50% of maximum. The EC50 in the above example is less than 0.6 ng/mL. (B) Human Recombinant EPO stimulates the proliferation and differentiation of erythroid progenitor cells, and was validated by titration in a CFU assay on human bone marrow MNCs, using MethoCult™ SF H4236 (Catalog #04236). The total number of colonies at each EPO concentration was enumerated using STEMvision™ (Catalog #22006). (C) 5 μg of Human Recombinant EPO was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant EPO polypeptide has a predicted molecular mass of 18.4 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 26 - 36 kDa in SDS-PAGE.