EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-2073_C
Gremlin-1，不含动物成分
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总览
Gremlin-1是一种分泌蛋白，特异性结合骨形态发生蛋白（BMP）家族蛋白，包括BMP-2和BMP-4，阻止其与其受体相互作用（Hsu等）。Gremlin-1在结肠基底隐窝中表达，它被认为有助于干细胞微环境的维持（Kosinski等）。Gremlin-1的表达水平在疾病中升高，包括一些结肠肿瘤（Kosinski等）、肝脏疾病和糖尿病（Baboota等）。此外，Gremlin-1的过表达已被证明以不依赖于VEGFR2和BMP的方式促进癌细胞的迁移、增殖和侵袭性（Kim等）。Gremlin-1 也被用于培养人结肠上皮组织和癌症类器官（Urbischek 等人）。本产品不含动物成分（ACF）。
亚型
细胞因子
别名
BMP 拮抗因子 1；细胞增殖诱导基因 2 蛋白；半胱氨酸结超家族 1；DAN 结构域家族成员 2；在 Mos 转化细胞中下调的蛋白；DRM
种属
人，其它细胞系
研究领域
癌症，上皮细胞研究
制剂类别
Animal Component-Free
纯度
SDS-PAGE 检测：≥ 98%
实验数据
Figure 1. Biological Activity and Molecular Mass of Human Recombinant Gremlin-1, ACF
(A) The biological activity of Human Recombinant Gremlin-1, ACF was tested by its ability to inhibit BMP-2 activity in stably transfected HEK293T cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity.The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.88 nM (34 ng/mL).
(B) 7 μg of Human Recombinant Gremlin-1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Gremlin-1, ACF has a predicted molecular mass of 18 kDa (monomer). The characteristic diffused bands are due to the dissociation of the non-covalent dimer during electrophoresis.
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Gremlin-1, ACF Catalog #
100-2073 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Gremlin-1, ACF Catalog #
100-2073 Lot #
All Language
English Request Pricing
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更多信息
| 种属 | Human, Other |
|---|---|
| 配方类别 | Animal Component-Free |
| Alternative Names | BMP antagonist 1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, DRM |
| 纯度 | ≥ 98% by SDS-PAGE |
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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