人重组R-Spondin-1（大肠杆菌表达），ACF

R-Spondin-1，无动物成分

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产品号 #100-1729_C

R-Spondin-1，无动物成分

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总览

实验数据

产品说明书及文档

应用领域

总览

R-Spondin-1 （RSPO1）是R-Spondin（RSPO）蛋白家族的原型成员，属于血小板反应蛋白1型重复序列（TSR-1）的蛋白超家族的亚家族（Chen et al. Mol Biol Rep, 2002; Kazanskaya et al. Dev Cell, 2004; Kim et al. Cell Cycle, 2006)。虽然RSPO家族成员本身不能启动信号通路，但却是WNT信号通路的强效增强因子（Cruciat & Niehrs. Cold Spring Harb Perspect Biol, 2013; de Lau et al. Genome Biol, 2012; Kazanskaya et al. Dev Cell, 2004）。该家族的结构特征是：包含一个TSR-1结构域、一个带正电氨基酸的羧基端区域和两个N端富含半胱氨酸的furin样重复结构（Glinka et al. EMBO Rep, 2012; Kazanskaya et al. Dev Cell, 2004）。RSPO1通过激活WNT信号级联反应中的β-连环蛋白（β-catenin）信号通路来发挥功能，它还能通过间接增加细胞表面的低密度脂蛋白受体相关蛋白6（LRP6）来增强信号。RSPO1与富含亮氨酸重复序列的G蛋白偶联受体5（LGR5）结合，并与WNT拮抗剂DKK-1竞争WNT共受体Kremen和LRP6的结合位点，从而减少DKK-1介导的LRP6内化作用（Binnerts et al. Proc Natl Acad Sci USA, 2007）。RSPO1在胚胎发生过程中参与了广泛的多向性功能。它在造血干细胞的命运决定中是必需的，并且已被证明在卵巢癌细胞的生长，存活和迁移中起重要作用（Cruciat & Niehrs. Cold Spring Harb Perspect Biol, 2013; de Lau et al. Genome Biol, 2012; Genthe & Clements. Development, 2017; Liu et al. Gene, 2019）。

亚型
细胞因子

别名
hRspo1，脊髓板特异性脊蛋白-1

细胞类型
内胚层，PSC衍生，上皮细胞，造血干/祖细胞，肠道细胞，中胚层，PSC衍生，其它细胞系，多能干细胞

种属
人

研究领域
上皮细胞研究，干细胞生物学

制剂类别
Animal Component-Free

纯度
≥98%

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实验数据

Figure 1. Biological Activity and Molecular Mass of Human Recombinant R-Spondin-1 (E. coli-expressed), ACF

(A) The biological activity of Human Recombinant R-Spondin-1 (E. coli-expressed), ACF was tested by its ability to induce luciferase activity in HEK239T cells transfected with the TOP-FLASH reporter. Luciferase activity was measured after cells were treated with increasing concentration of R-spondin-1 (diluted in DMEM with 0.5% of fetal calf serum), in the presence of Wnt-conditioned medium (1 in 8 dilution). The EC50 is defined as the effective concentration of the growth factor at which Wnt-ß catenin activity is at 50% of maximum. The EC50 in the above example is 5.8 nM (75.1 ng/mL). (B) 7 µg of Human Recombinant R-Spondin-1 (E. coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant R-Spondin-1 has a predicted molecular mass of 13 kDa in reducing conditions and 16 kDa in non-reducing conditions.