EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-1797_C
转化生长因子β 1，无动物成分
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总览
转化生长因子β1 （TGF-β1）是TGF-β超家族的成员，调控多种细胞表型。该产品使用LAL法测定，内毒素水平≤0.1 EU/μg，可以放心地在多种应用中使用。TGF-β1通过与I型和II型丝氨酸-苏氨酸激酶受体结合，激活SMAD2/3蛋白介导的信号通路，从而调节细胞增殖、分化、伤口愈合、凋亡和代谢等(Massagué; McDowell et al.)。在人胚胎发育过程中，TGF-β1由内皮和造血组织表达，并作为一种内源性的自分泌生长调节剂（Gatherer et al.）。TGF-β1与碱性成纤维细胞生长因子（bFGF）一起可支持未分化的人胚胎干细胞和诱导多能干细胞的体外培养（Amit et al.）。TGF-β1激活后 ，可通过通过调节 B 细胞反应及对 T 细胞和中性粒细胞的免疫抑制作用，维持免疫系统稳态（Letterio & Roberts）。本产品为动物成分（ACF）级别。
亚型
细胞因子
别名
软骨诱导因子，分化抑制因子，LAP（潜伏相关肽），TGFB1，TGF-β1，转化生长因子β1
细胞类型
B 细胞，间充质干/祖细胞，单核细胞，髓系细胞，神经干/祖细胞，多能干细胞，T 细胞，调节性细胞
种属
人，其它细胞系
研究领域
免疫，神经科学，干细胞生物学
制剂类别
Animal Component-Free
纯度
SDS-PAGE 检测：≥ 98%
实验数据
Figure 1. Biological Activity and Molecular Mass of Human Recombinant TGF-beta 1, ACF
(A) The biological activity of Human Recombinant TGF-beta 1, ACF was tested using a luciferase reporter assay in transiently transfected HEK293T cells. Firefly luciferase activity was normalized to control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which TGF beta 1 response is at 50% of maximum. The EC50 in the above example is 1.54 pM (~0.04 ng/mL).
(B) 7 μg of Human Recombinant TGF-beta 1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TGF-beta 1, ACF has a predicted molecular mass of 24 kDa (dimer).
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant TGF-beta 1, ACF Catalog #
100-1798 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant TGF-beta 1, ACF Catalog #
100-1798 Lot #
All Language
English 应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
Research Area
Workflow Stages
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Human Pluripotent Stem Cell Research
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更多信息
| 种属 | Human, Other |
|---|---|
| 配方类别 | Animal Component-Free |
| Alternative Names | Cartilage-inducing factor, Differentiation inhibiting factor, LAP, Latency-associated peptide, TGFB1, TGF-beta-1, Transforming growth factor β 1 |
| 纯度 | ≥ 98% by SDS-PAGE |
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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