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重组人TGF-β1（即用型）

即用型转化生长因子β1
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产品号 #100-0856_C

即用型转化生长因子β1

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总览
实验数据
产品说明书及文档
应用领域
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总览

使用这款细胞培养即用型配方，用户可以方便且一致地将转化生长因子β1（TGF-ꞵ1）添加到培养物中。该重组人TGF-ꞵ1可在 100 mM 乙酸中精确配制至 0.1 mg/mL，无需额外制备，从而提高可重复性。采用LAL方法验证内毒素水平，以确保在多种应用中的一致性。作为TGF-β超家族的成员，TGF-β1可调节多种细胞表型。TGF-β1与丝氨酸-苏氨酸激酶 I 型和 II 型受体结合，并通过SMAD2/3蛋白激活信号转导。

TGF-β1可用于：
hPSC维持培养
●搭配碱性成纤维细胞生长因子（bFGF），支持未分化人胚胎干细胞（hESC）和诱导多能干细胞（hiPSC）的培养（Amit et al. Biol Reprod, 2004）。
外源细胞信号
●调节多种功能，包括细胞扩增、分化、伤口愈合、凋亡和代谢（Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997）。
免疫细胞稳态
●调节B细胞反应并介导对T细胞和中性粒细胞的免疫抑制作用（Letterio & Roberts Annu Rev Immunol, 1998）。

别名
软骨诱导因子，分化抑制因子，TGF-beta-1，TGF-β1，转化生长因子β1
 
细胞类型
B 细胞，间充质干/祖细胞，单核细胞，髓系细胞，神经干/祖细胞，多能干细胞，T 细胞，调节性细胞
 
种属
人
 
研究领域
免疫，神经科学，干细胞生物学
 
纯度
SDS-PAGE 检测：≥ 95%
 

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实验数据

Figure 1. Human Recombinant TGF-beta 1 Is Bioactive

The biological activity of Human Recombinant TGF-beta 1 was tested by its ability to induce luciferase expression in a SMAD/TGF luciferase reporter cell line. The EC50 is defined as the effective concentration of the agonist at which luminescence is 50% of maximum. The EC50 in the example above is 0.52 ng/mL. Normalized mean % of 3 replicate wells is shown for each data point. Each assay is calibrated with a Non-WHO International Reference Material (NIBSC code: 89/514). EC50 is assay-specific; titrations should be performed to determine the optimal concentration for the intended application.

Figure 2. Human Recombinant TGF-beta 1 Has High Purity

Human Recombinant TGF-beta 1 (1 μg) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Blue staining. Human Recombinant TGF-beta 1 is a homodimer of 12.8-kDa subunits with a predicted molecular mass of 25.6 kDa.

Sources of TGF-beta 1 from STEMCELL Technologies and a leading competitor demonstrate similar biological activity in the luciferase assay

Figure 3. Sources of TGF-beta 1 from STEMCELL Technologies and a leading competitor demonstrate similar biological activity in the luciferase assay

The biological activity of TGF-beta 1 was determined using a luciferase reporter cell line responsive to SMAD2/3. Luciferase activity was measured 24 hours after application of the TGF-beta 1 dilution series to the cells and the EC50 was determined by four-parameter curve fitting. EC50 values of the three TGF-beta 1 sources were: Hu TGF-beta 1(Cell Culture Ready) = 1.182 ng/mL; Hu TGF-beta 1 = 1.109 ng/mL; Competitor TGF-beta 1 = 1.649 ng/mL. The EC50 is defined as the effective concentration of the growth factor at which relative luminescence is 50% of maximum. There were no significant differences in the EC50 values between samples, and all were within the normal biological variability of the assay.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Recombinant TGF-beta 1 (Cell Culture Ready)
Catalog #
100-0856
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant TGF-beta 1 (Cell Culture Ready)
Catalog #
100-0856
Lot #
All
Language
English

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更多信息
种属 Human
Alternative Names Cartilage-inducing factor, Differentiation inhibiting factor, TGF-beta-1, TGF-ꞵ1, Transforming growth factor beta 1
纯度 ≥ 95% by SDS-PAGE
质量保证:

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