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ImmunoCult™-ACF树突状细胞培养基

用于人单核细胞培养、往树突状细胞分化及成熟的培养基
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产品号 #10986_C

用于人单核细胞培养、往树突状细胞分化及成熟的培养基

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总览
实验数据
产品说明书及文档
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总览

使用无血清且不含动物成分的ImmunoCult™-ACF树突状细胞培养基，可稳定将人单核细胞并培养并分化为树突状细胞（DC）。

该培养基作为ImmunoCult™树突状细胞培养试剂盒的组分，也可单独购买，以方便您的使用。将ImmunoCult™-ACF树突状细胞培养基与ImmunoCult™-ACF树突状细胞分化添加物搭配使用，可在体外生成DC；当与ImmunoCult™树突状细胞成熟添加物搭配使用时，可支持未成熟DC转化为成熟DC。

关于使用ImmunoCult™树突状细胞培养基进行培养和分化的详细方法，请参阅产品说明书（PIS）。

该培养基作为ImmunoCult™树突状细胞培养试剂盒的组分，也可单独购买，以方便您的使用。将ImmunoCult™-ACF树突状细胞培养基与ImmunoCult™-ACF树突状细胞分化添加物搭配使用，可在体外生成DC；当与ImmunoCult™树突状细胞成熟添加物搭配使用时，可支持未成熟DC转化为成熟DC。

关于使用ImmunoCult™树突状细胞培养基进行培养和分化的详细方法，请参阅产品说明书（PIS）。

该培养基作为ImmunoCult™树突状细胞培养试剂盒的组分，也可单独购买，以方便您的使用。将ImmunoCult™-ACF树突状细胞培养基与ImmunoCult™-ACF树突状细胞分化添加物搭配使用，可在体外生成DC；当与ImmunoCult™树突状细胞成熟添加物搭配使用时，可支持未成熟DC转化为成熟DC。

关于使用ImmunoCult™树突状细胞培养基进行培养和分化的详细方法，请参阅产品说明书（PIS）。

该培养基作为ImmunoCult™树突状细胞培养试剂盒的组分，也可单独购买，以方便您的使用。将ImmunoCult™-ACF树突状细胞培养基与ImmunoCult™-ACF树突状细胞分化添加物搭配使用，可在体外生成DC；当与ImmunoCult™树突状细胞成熟添加物搭配使用时，可支持未成熟DC转化为成熟DC。

关于使用ImmunoCult™树突状细胞培养基进行培养和分化的详细方法，请参阅产品说明书（PIS）。

该培养基作为ImmunoCult™树突状细胞培养试剂盒的组分，也可单独购买，以方便您的使用。将ImmunoCult™-ACF树突状细胞培养基与ImmunoCult™-ACF树突状细胞分化添加物搭配使用，可在体外生成DC；当与ImmunoCult™树突状细胞成熟添加物搭配使用时，可支持未成熟DC转化为成熟DC。

关于使用ImmunoCult™树突状细胞培养基进行培养和分化的详细方法，请参阅产品说明书（PIS）。

亚型
专用培养基，添加剂
 
细胞类型
树突状细胞（DCs），单核细胞
 
种属
人
 
应用
细胞培养，分化
 
品牌
ImmunoCult
 
研究领域
免疫
 
制剂类别
Animal Component-Free，无血清
 

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实验数据

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 1. Protocol Diagram.

Mature DCs were generated by culturing EasySep™ isolated monocytes at 1 x 106 cells/mL in ImmunoCult™-ACF Dendritic Cell Medium (Catalog #10987) with added ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (Catalog #10988). At day 3, the medium with differentiation supplement was replaced and cells were incubated for 2 more days. At day 5, without changing the medium, ImmunoCult™ Dendritic Cell Maturation Supplement (Catalog #10989) was added to the culture. At day 7, fully mature DCs were harvested for downstream applications.

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 2. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium with Supplements show desired phenotype.

EasySep™ isolated monocytes were cultured and differentiated into mature DCs as described in Figure 1. (A) The percentage of CD14 and CD83 expression in cells at day 7 (mature DCs) was determined by flow cytometry. At day 7, a total of 93 ± 5% of the cells expressed the mature DC marker CD83 and only 1 ± 1% of cells still expressed the monocyte marker CD14 (mean ± SD, n=39). Yield of mature DCs was determined by count of total viable cells at day 7 relative to the count of viable monocytes used for initial culture at day 0. At day 7, the yield of viable mature DCs corresponded to 45 ± 25% (mean ± SD, n=39). (B) Immature DCs were cultured as described in Figure 1. At day 5, cells were cultured with maturation supplement for 2 days (mature DCs) or without maturation supplement (immature DCs). Supernatant was collected at day 7 and IL-12p70 levels were determined by ELISA. Concentrations of IL-12p70 in supernatant of mature and immature DCs were 361 ± 81 and 5 ± 2 pg/mL, respectively (mean ± SEM, n=27).

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 3. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements induce T cell proliferation.

Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements (ImmunoCult) or other serum-free competitor media (competitor 1 and 2) and corresponding supplements when applicable (competitor 2), were cultured in ImmunoCult™-XF T Cell Expansion Medium with 1 x 105 CFSE labeled (A) allogeneic CD3+ T cells (MLR assay) or (B) autologous CD8+ T cells (antigen-specific T cell response). (A) Cells were cultured at a DC:T cell ratio of 1:25. (B) Prior to culture with T cells, immature DCs were loaded with HLA Class I peptides derived from the human Cytomegalovirus, Epstein-Barr Virus and Influenza Virus (CEF peptide pool) and stimulated with maturation supplement for 2 days. Cells were cultured at a DC:T cell ratio of 1:4 or 1:10. (A,B) CFSE labeled T cells were incubated in media alone (negative control) or with ImmunoCult™ Human CD3/CD28 T Cell Activator (positive control). After 5-7 days in culture the number of dividing T cells ( CD3+CFSElo) was assessed by flow cytometry (mean ± SEM) (A) n=5 (B) n=4 (competitor 1 and 2, n=3). Mature DCs generated in ImmunoCult™-ACF Dendritic Cell Medium induced proliferation of allogeneic and antigen-specific T cells similar to DCs generated in either competitor media. Competitors 1 and 2, include in no particular order, CellGro DC Medium (CellGenix) and PromoCell DC Generation Medium DXF.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Medium
Catalog #
10986, 10987
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Dendritic Cell Medium
Catalog #
10986, 10987
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

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种属 Human
配方类别 Animal Component-Free, Serum-Free
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