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ImmunoCult™ 树突状细胞培养试剂盒

人单核细胞向树突状细胞分化的完整试剂盒
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产品号 #（选择产品）

产品号 #10985_C

人单核细胞向树突状细胞分化的完整试剂盒

产品优势

  • 无动物源成分和无血清培养基
  • 无需在培养基中添加血清
  • 针对CD14+单核细胞分化为未成熟和成熟的树突状细胞（DC）进行了优化
  • 所需表型的成熟树突状细胞（DC）的高得率
  • 成熟的树突状细胞（DC）具有功能性，可产生促炎细胞因子IL-12，并诱导T细胞增殖

产品组分包括

  • ImmunoCult™-ACF树突状细胞基础培养基，100 mL（产品号 #10987）
  • ImmunoCult™-ACF树突状细胞分化添加剂，1 mL（产品号 #10988）
  • ImmunoCult™树突状细胞成熟添加剂，0.5 mL（产品号 #10989）
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献

总览

使用无动物源成分 (ACF) ImmunoCult™ 树突状细胞培养试剂盒，培养并分化人单核细胞为树突状细胞 (DC)。

为了方便起见，该试剂盒包含所有必需成分，可帮助您在 7 天内从人单核细胞中诱导出具有良好活性及功能性的成熟的树突状细胞 (DC)：

• ImmunoCult™-ACF 树突状细胞培养基，用于体外培养和分化人单核细胞为树突状细胞 (DC)。

• ImmunoCult™-ACF 树突状细胞分化添加剂，用于支持人单核细胞分化成未成熟树突状细胞。

• ImmunoCult™ 树突状细胞成熟添加剂，用于支持未成熟人树突状细胞的成熟。

有关使用 ImmunoCult™ 树突状细胞培养试剂盒进行培养和分化的更多方案信息，请参阅产品信息表 (PIS)。

为了方便起见，该试剂盒包含所有必需成分，可帮助您在 7 天内从人单核细胞中诱导出具有良好活性及功能性的成熟的树突状细胞 (DC)：

• ImmunoCult™-ACF 树突状细胞培养基，用于体外培养和分化人单核细胞为树突状细胞 (DC)。

• ImmunoCult™-ACF 树突状细胞分化添加剂，用于支持人单核细胞分化成未成熟树突状细胞。

• ImmunoCult™ 树突状细胞成熟添加剂，用于支持未成熟人树突状细胞的成熟。

有关使用 ImmunoCult™ 树突状细胞培养试剂盒进行培养和分化的更多方案信息，请参阅产品信息表 (PIS)。

为了方便起见，该试剂盒包含所有必需成分，可帮助您在 7 天内从人单核细胞中诱导出具有良好活性及功能性的成熟的树突状细胞 (DC)：

• ImmunoCult™-ACF 树突状细胞培养基，用于体外培养和分化人单核细胞为树突状细胞 (DC)。

• ImmunoCult™-ACF 树突状细胞分化添加剂，用于支持人单核细胞分化成未成熟树突状细胞。

• ImmunoCult™ 树突状细胞成熟添加剂，用于支持未成熟人树突状细胞的成熟。

有关使用 ImmunoCult™ 树突状细胞培养试剂盒进行培养和分化的更多方案信息，请参阅产品信息表 (PIS)。

为了方便起见，该试剂盒包含所有必需成分，可帮助您在 7 天内从人单核细胞中诱导出具有良好活性及功能性的成熟的树突状细胞 (DC)：

• ImmunoCult™-ACF 树突状细胞培养基，用于体外培养和分化人单核细胞为树突状细胞 (DC)。

• ImmunoCult™-ACF 树突状细胞分化添加剂，用于支持人单核细胞分化成未成熟树突状细胞。

• ImmunoCult™ 树突状细胞成熟添加剂，用于支持未成熟人树突状细胞的成熟。

有关使用 ImmunoCult™ 树突状细胞培养试剂盒进行培养和分化的更多方案信息，请参阅产品信息表 (PIS)。

包含
ImmunoCult™-ACF Dendritic Cell Medium contains only recombinant proteins and synthetic components
 
亚型
专用培养基，添加剂
 
细胞类型
树突状细胞（DCs），单核细胞
 
种属
人
 
应用
细胞培养，分化
 
品牌
ImmunoCult
 
研究领域
免疫
 
制剂类别
Animal Component-Free，无血清
 

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实验数据

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 1. Protocol Diagram.

Mature DCs were generated by culturing EasySep™ isolated monocytes at 1 x 106 cells/mL in ImmunoCult™-ACF Dendritic Cell Medium (Catalog #10987) with added ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (Catalog #10988). At day 3, the medium with differentiation supplement was replaced and cells were incubated for 2 more days. At day 5, without changing the medium, ImmunoCult™ Dendritic Cell Maturation Supplement (Catalog #10989) was added to the culture. At day 7, fully mature DCs were harvested for downstream applications.

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 2. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium with Supplements show desired phenotype.

EasySep™ isolated monocytes were cultured and differentiated into mature DCs as described in Figure 1. (A) The percentage of CD14 and CD83 expression in cells at day 7 (mature DCs) was determined by flow cytometry. At day 7, a total of 93 ± 5% of the cells expressed the mature DC marker CD83 and only 1 ± 1% of cells still expressed the monocyte marker CD14 (mean ± SD, n=39). Yield of mature DCs was determined by count of total viable cells at day 7 relative to the count of viable monocytes used for initial culture at day 0. At day 7, the yield of viable mature DCs corresponded to 45 ± 25% (mean ± SD, n=39). (B) Immature DCs were cultured as described in Figure 1. At day 5, cells were cultured with maturation supplement for 2 days (mature DCs) or without maturation supplement (immature DCs). Supernatant was collected at day 7 and IL-12p70 levels were determined by ELISA. Concentrations of IL-12p70 in supernatant of mature and immature DCs were 361 ± 81 and 5 ± 2 pg/mL, respectively (mean ± SEM, n=27).

Start: 54% CD4+CXCR3-CCR6+ T Cells

Figure 3. Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements induce T cell proliferation.

Mature DCs generated with ImmunoCult™-ACF Dendritic Cell Medium and Supplements (ImmunoCult) or other serum-free competitor media (competitor 1 and 2) and corresponding supplements when applicable (competitor 2), were cultured in ImmunoCult™-XF T Cell Expansion Medium with 1 x 105 CFSE labeled (A) allogeneic CD3+ T cells (MLR assay) or (B) autologous CD8+ T cells (antigen-specific T cell response). (A) Cells were cultured at a DC:T cell ratio of 1:25. (B) Prior to culture with T cells, immature DCs were loaded with HLA Class I peptides derived from the human Cytomegalovirus, Epstein-Barr Virus and Influenza Virus (CEF peptide pool) and stimulated with maturation supplement for 2 days. Cells were cultured at a DC:T cell ratio of 1:4 or 1:10. (A,B) CFSE labeled T cells were incubated in media alone (negative control) or with ImmunoCult™ Human CD3/CD28 T Cell Activator (positive control). After 5-7 days in culture the number of dividing T cells ( CD3+CFSElo) was assessed by flow cytometry (mean ± SEM) (A) n=5 (B) n=4 (competitor 1 and 2, n=3). Mature DCs generated in ImmunoCult™-ACF Dendritic Cell Medium induced proliferation of allogeneic and antigen-specific T cells similar to DCs generated in either competitor media. Competitors 1 and 2, include in no particular order, CellGro DC Medium (CellGenix) and PromoCell DC Generation Medium DXF.

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
ImmunoCult™ Dendritic Cell Culture Kit
Catalog #
10985
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human
Contains ImmunoCult™-ACF Dendritic Cell Medium contains only recombinant proteins and synthetic components
配方类别 Animal Component-Free, Serum-Free
质量保证:

产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE.
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