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ImmunoCult™ 人Th1分化添加剂

人naïve CD4+ T细胞向Th1细胞分化的无血清培养添加剂
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产品号 #10973_C

人naïve CD4+ T细胞向Th1细胞分化的无血清培养添加剂

产品优势

  • 专为诱导人Th1细胞分化而配制
  • 经优化可搭配使用ImmunoCult™-XF T细胞扩增培养基
  • 较竞品Th1分化试剂盒相比，体现更高占比的记忆T细胞
  • 较竞品Th1分化试剂盒相比，产IFN-γ的CD4+ T细胞的扩增数量高达1.6倍的
  • 以50倍浓缩液形式提供；解冻并混匀后，管内容物可直接添加至培养基中
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

  • ImmunoCult™人Th1分化添加剂含有多种人源细胞因子和一种封闭抗体，旨在促进外周血来源的人初始CD4+T细胞高效稳定激活、扩增并分化为Th1细胞。ImmunoCult™人Th1分化添加剂可与ImmunoCult™-XF T细胞扩增培养基（产品号 #10981）和ImmunoCult™人CD3/CD28 T细胞激活剂（产品号 #10971）搭配使用。
  • 本产品推荐用于：
  • •研究人初始T细胞分化和Th1功能的调节
  • •开发初始T细胞扩增及向Th1亚群极化的工艺流程

包含
• Recombinant human interleukin 12 (IL-12) • Recombinant human interleukin 18 (IL-18) • Mouse anti-human interleukin 4 (anti-IL-4)
 
亚型
添加剂
 
细胞类型
T 细胞，T 细胞，CD4+
 
种属
人
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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实验数据

Figure 1. ImmunoCult™ Human Th1 Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Th1 Differentiation Supplement Produces More Differentiated CD4+CD45RA-CD45RO+ Cells Under Th1 Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th1 Differentiation Supplement (Catalog #10973) in ImmunoCult™ and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype was significantly lower and the percentage of cells expressing a differentiated phenotype was significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p < 0.05; paired t-test on logit transformed data; n = 4).

Figure 3. Culture with ImmunoCult™ Human Th1 Differentiation Supplement Produces More IFNγ-Expressing Cells Than Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. After culture, differentiated CD4+ T cells (CD45RA-CD45RO+) were analyzed for IFNγ and IL-4 (not shown) expression, following stimulation with PMA and Ionomycin for 4 hours. A greater percentage of cells generated with ImmunoCult™ expressed intracellular IFNγ compared to those generated under competitor conditions, while no significant difference was found in the percentage of cells which expressed intracellular IL-4, with very low numbers of cells expressing IL-4 for both ImmunoCult™ and competitor. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p< 0.05; paired t-test on logit transformed data; n = 4).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Human Th1 Differentiation Supplement
Catalog #
10973
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Human Th1 Differentiation Supplement
Catalog #
10973
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
种属 Human
Contains • Recombinant human interleukin 12 (IL-12) • Recombinant human interleukin 18 (IL-18) • Mouse anti-human interleukin 4 (anti-IL-4)
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