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ImmunoCult™ 人Th2分化添加剂

人naïve CD4+ T细胞向Th2细胞分化的无血清培养添加剂
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产品号 #10975_C

人naïve CD4+ T细胞向Th2细胞分化的无血清培养添加剂

产品优势

  • 专为诱导人Th2细胞分化而配制
  • 经优化可搭配使用ImmunoCult™-XF T细胞扩增培养基
  • 较竞品Th2分化试剂盒相比，体现更高占比的记忆T细胞
  • 较竞品Th2分化试剂盒相比，产IL-4的CD4+ T细胞的扩增数量高达3倍
  • 以50倍浓缩液形式提供；解冻并混匀后，管内容物可直接添加至培养基中
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

ImmunoCult™人Th2分化添加剂含有人源细胞因子和封闭抗体，旨在促进外周血来源的人初始CD4+T细胞高效稳定激活、扩增并分化为Th2细胞。ImmunoCult™人Th2分化添加剂可与ImmunoCult™-XF T细胞扩增培养基（产品号 #10981）和ImmunoCult™人CD3/CD28 T细胞激活剂（产品号 #10971）搭配使用。

本产品推荐用于：

•研究人初始T细胞分化和Th2功能的调节

•开发初始T细胞扩增及向Th2亚群极化的工艺流程

本产品推荐用于：

•研究人初始T细胞分化和Th2功能的调节

•开发初始T细胞扩增及向Th2亚群极化的工艺流程

本产品推荐用于：

•研究人初始T细胞分化和Th2功能的调节

•开发初始T细胞扩增及向Th2亚群极化的工艺流程

本产品推荐用于：

•研究人初始T细胞分化和Th2功能的调节

•开发初始T细胞扩增及向Th2亚群极化的工艺流程

包含
• Recombinant human interleukin 4 (IL-4) • Mouse anti-human interferon-gamma (anti-IFN-γ)
 
亚型
添加剂
 
细胞类型
T 细胞，CD4+
 
种属
人
 
应用
细胞培养，分化，扩增
 
品牌
ImmunoCult
 
研究领域
免疫
 

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实验数据

Figure 1. ImmunoCult™ Human Th2 Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Th2 Polarizing Conditions

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th2 Differentiation Supplement (Catalog #10975), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 14 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 14 days of culture. After 14 days the majority of cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).

Figure 2. ImmunoCult™ Human Th2 Differentiation Supplement Produces More Differentiated CD4+CD45RA-CD45RO+ Cells Under Th2 Polarizing Conditions Than Competitor

Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Th2 Differentiation Supplement (Catalog #10975) in ImmunoCult™ and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype was similar and the percentage of cells expressing a differentiated phenotype was significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and mature T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; *p < 0.05; paired t-test on logit transformed data; n = 4).

Figure 3. Culture with ImmunoCult™ Human Th2 Differentiation Supplement Produces Similar Percentages of Cells Expressing IFNγ and IL-4 as Culture Under Competitor Conditions

Naïve CD4+ T cells were cultured for 13 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. After culture, differentiated CD4+ T cells (CD45RA-CD45RO+) were analyzed for (A) IFNγ and (B) IL-4 expression, following stimulation with PMA and Ionomycin for 4 hours. Similar percentages of cells generated with ImmunoCult™ expressed intracellular IFNγ and IL-4 as compared to those generated under competitor conditions. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Human Th2 Differentiation Supplement
Catalog #
10975
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Human Th2 Differentiation Supplement
Catalog #
10975
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

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种属 Human
Contains • Recombinant human interleukin 4 (IL-4) • Mouse anti-human interferon-gamma (anti-IFN-γ)
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