EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #10977_C
人naïve CD4+ T细胞向调节性T细胞（Tregs）分化的无血清培养添加剂
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总览
ImmunoCult™人Treg分化添加剂含有人源细胞因子和小分子，旨在促进外周血来源的人初始CD4+T细胞高效稳定激活、扩增并分化为Treg细胞。ImmunoCult™人Th2分化添加剂可与ImmunoCult™-XF T细胞扩增培养基（产品号 #10981）和ImmunoCult™人CD3/CD28 T细胞激活剂（产品号 #10971）搭配使用。
本产品推荐用于：
•研究人初始T细胞分化和Treg功能的调节
•开发初始T细胞扩增及向Treg极化的工艺流程
本产品推荐用于：
•研究人初始T细胞分化和Treg功能的调节
•开发初始T细胞扩增及向Treg极化的工艺流程
本产品推荐用于：
•研究人初始T细胞分化和Treg功能的调节
•开发初始T细胞扩增及向Treg极化的工艺流程
本产品推荐用于：
•研究人初始T细胞分化和Treg功能的调节
•开发初始T细胞扩增及向Treg极化的工艺流程
包含
• Recombinant human TGF-β1
• All-trans retinoic acid
亚型
添加剂
细胞类型
T 细胞，T 细胞，CD4+
种属
人
应用
细胞培养，分化，扩增
研究领域
免疫
实验数据
Figure 1. ImmunoCult™ Human Treg Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of Treg cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).
Figure 2. ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Numbers of Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions Than Competitor
Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977) in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) (purple), or competitor medium and activator (grey) for 5 days (based on the length of the competitor protocol). The percentage of naïve (CD45RA+CD45RO-) and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype and the percentage of cells expressing a differentiated phenotype were not significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4).
Figure 3. ImmunoCult™ Human Treg Differentiation Supplement Produces FOXP3+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were cultured for 7 days in ImmunoCult™ activator, supplement and medium using the protocol described in Figure 2. Cells were stained for CD4, CD4, CD25 and FOXP3 and analyzed on days 0, 4, 5 and 7 by flow cytometry. Each bar represents the mean percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) (mean ± S.E.M; n = 4 – 20 donors).
Figure 4. Culture with ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Percentages of CD3+CD4+CD25+FOXP3+ Cells as Culture Under Competitor Conditions
Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. The percentage of CD25+FOXP3+ after 5 days of culture was compared after staining cells for CD3, CD4, CD25 and FOXP3 and analysis by flow cytometry. Similar percentages of CD3+CD4+ cells generated with ImmunoCult™ expressed CD25 and FOXP3 as compared to those generated under competitor conditions. Shown are the differences in the percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4 - 20).
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
ImmunoCult™ Human Treg Differentiation Supplement Catalog #
10977 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
ImmunoCult™ Human Treg Differentiation Supplement Catalog #
10977 Lot #
All Language
English 应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
Research Area
Workflow Stages
相关材料与文献
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更多信息
| 种属 | Human |
|---|---|
| Contains | • Recombinant human TGF-β1 • All-trans retinoic acid |
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
产品仅供研究使用，不用于针对人或动物的诊断或治疗。 欲获悉更多关于STEMCELL的质控信息，请访问 STEMCELL.CN/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
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