ImmunoCult™ 小鼠B细胞扩增试剂盒

用于小鼠B细胞体外扩增的无血清培养试剂盒

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产品号 #（选择产品）

产品号 #100-1003_C

用于小鼠B细胞体外扩增的无血清培养试剂盒

产品优势

无需使用血清、饲养细胞或专用培养板，即可实现小鼠B细胞的稳定体外扩增
使用优化且易于操作的培养体系，简化实验流程
获得稳定高效扩增的小鼠B细胞

产品组分包括

ImmunoCult™-XF B细胞基础培养基，100 mL（产品号 #100-0646）；
ImmunoCult™-ACF小鼠B细胞扩增添加剂，2ml（产品号 #100-1004）

总览

实验数据

产品说明书及文档

应用领域

总览

在无血清条件下持续扩增小鼠B细胞，无需使用饲养细胞。

ImmunoCult™小鼠B细胞扩增试剂盒可提供优化的培养条件，实现高倍数地扩增小鼠B细胞。该试剂盒包含ImmunoCult™-XF B细胞基础培养基和ImmunoCult™ACF小鼠B细胞扩增添加剂，为您提供强大且易于操作的培养系统。仅需培养6 - 9天，即可收获细胞并直接用于下游应用。

该试剂盒与我们其它上游和下游产品兼容。例如，您可以使用EasySep™小鼠Pan-B细胞分选试剂盒分离B细胞，分选后可立即使用ImmunoCult™小鼠B细胞扩增试剂盒进行扩增培养。

分类
专用培养基

细胞类型
B 细胞

种属
小鼠

应用
细胞培养，扩增

品牌
ImmunoCult

研究领域
抗体制备，免疫，感染性疾病(传染病)，细胞治疗开发

制剂类别
不含动物成分，无血清，无异源

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实验数据

Figure 1. Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured in complete Mouse B Cell Expansion Medium as described in Directions For Use (steps 1 - 7). B Cells were harvested on day 9 for analysis. Cells can also be harvested at earlier time points depending on different applications.

Figure 2. Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Fold expansion of viable cells is shown with bar graphs representing the mean ± SEM (n = 8). B cells expanded 176.9 ± 29.8-fold after 9 days of culture.

Figure 3. Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Following staining using the protocol by Pracht et al. (Eur J Immunol, 2017), the expression of A) B220 and CD138 and B) TACI (CD267) and CD86 were analyzed by flow cytometry at several time points (data represents mean ± SEM, n = 8). An increase in CD86 cell surface expression indicates B cell activation; a decrease in B220 and an increase in CD138 and TACI cell surface expression indicate maturation of B cells to plasmablasts or plasma cells.

Growth of Mouse B Cells When Cultured with ImmunoCult™ Mouse B Cell Expansion Kit Over the Span of 9 Days

Figure 4. Robust Growth of Mouse B Cells when Cultured with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Cells were imaged at A) 10X magnification on day 0, B) 10X magnification on day 3, C) 40X magnification on day 6, and D) 40X magnification on day 9.