ImmunoCult™-XF B细胞基础培养基

用于扩增B细胞的无血清和无异种成分基础培养基

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产品号 #（选择产品）

产品号 #100-0646_C

用于扩增B细胞的无血清和无异种成分基础培养基

产品优势

无需血清、饲养层细胞或专用培养板即可实现人B细胞的稳健体外扩增
无血清且无异种成分培养基

产品组分包括

ImmunoCult™-XF B细胞基础培养基，100 mL

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总览

实验数据

产品说明书及文档

应用领域

总览

使用无血清且无异种成分的ImmunoCult™-XF B细胞基础培养基支持人B细胞扩增。

该基础培养基作为ImmunoCult™人B细胞扩增试剂盒的组分，也可单独购买，需与ImmunoCult™-ACF人B细胞扩增添加物搭配使用，以配制用于培养和扩增人B细胞的无血清完全培养基。

如需了解更多关于使用ImmunoCult™培养基扩增B细胞的信息，请查阅产品说明书。

亚型
专用培养基

细胞类型
B 细胞

种属
人

应用
激活，细胞培养，分化，扩增，基因组编辑

品牌
ImmunoCult

研究领域
抗体制备，免疫，感染性疾病(传染病)

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实验数据

Fold Expansion and Maturation of B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy Image of Human B Cells Expanded In Vitro Without the Use of Serum, Feeder Cells, or Specialized Culture Plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit

Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.