Some of the greatest hurdles to the successful application of CRISPR-Cas technology in the clinic include suboptimal specificity, activity, and targeting range. In this webinar, Dr. Benjamin Kleinstiver presents a brief overview of genome editing and CRISPR technology. He then describes his lab's efforts to engineer enhanced CRISPR-Cas nucleases for improved genome editing specificity and discusses how these technologies can translate to more efficient tools and disease treatments.