IntestiCult™-SF 类器官生长培养基 (人)

用于构建和维持人肠类器官的无血清的非条件培养基

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产品号 #（选择产品）

产品号 #100-0340_C

用于构建和维持人肠类器官的无血清的非条件培养基

产品优势

便捷的体外培养系统，能重现成年肠上皮的多种关键特征，包括细胞内与细胞间信号传导、自我维持的干细胞微环境，以及在肠腔内外的功能性转运
无血清且非条件培养基的配方
适用于基因组编辑的实验方案
可在一周内生成肠类器官
完整的培养基配方，确保结果的一致性

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Interested in trying STEMCELL’s organoid products for your intestinal research? Fill out the form to request information about introductory offers.

要查看实验方案所需的所有配套产品，请参阅《实验方案与技术文档》。

Gentle Cell Dissociation Reagent
cGMP, enzyme-free cell dissociation reagent
Organoid Culture Plates
Cell culture plates for easy and reproducible generation of organoids
DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Y-27632 (Dihydrochloride)
RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

IntestiCult™-SF 类器官生长培养基 (人)是一套完整的培养基，用于从人的肠隐窝高效地构建肠类器官并对其进行长期的维持培养。IntestiCult™-SF 是无血清且非条件培养基的配方。

肠类器官为研究肠道上皮提供了一个便捷的体外器官型培养系统。分离的肠隐窝在 IntestiCult™-SF 中培养时能快速形成复杂类器官。该类器官包含一个由具有极性的肠上皮细胞层包围而成的功能性腔体结构，细胞层中包含已知的成年肠上皮细胞类型。成熟的肠类器官会形成突出的芽体，与体内组织的肠隐窝结构相似。IntestiCult™-SF 支持患者活检样本中肠上皮干细胞的增殖，以及新鲜组织或现有冻存样本来源的类器官的扩增。

肠类器官培养的应用包括研究肠道上皮的发育和功能、构建肠道疾病模型，以及在肠道模型中筛选分子的有效性和毒性。肠类器官培养还可用于研究成体干细胞特性及再生治疗方法，并可通过 IntestiCult™ 类器官分化培养基 (人)（产品号 #100-0214）实现进一步分化。

欢迎通过我们的免费点播课程学习人肠类器官的培养方法，或浏览关于使用 IntestiCult™ 进行类器官培养流程的常见问题（FAQs）。此外，您还可以下载我们的电子书《Proven Protocols for Intestinal Organoid Culture: Getting Started with IntestiCult™》，获取肠类器官培养方案的精选合集。

如果您打算将此产品用于商业目的，请通过www.huborganoids.nl联系HUB以获得商业使用许可或有关HUB许可的说明。

亚型
专用培养基

细胞类型
肠道细胞

种属
人

应用
类器官培养

品牌
IntestiCult

研究领域
疾病建模，药物发现和毒理检测，上皮细胞研究，类器官，干细胞生物学

制剂类别
无血清

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实验数据

Figure 1. Organoids Grown in IntestiCult™-SF Have a Comparable Morphology to Organoids Grown in IntestiCult Organoid Growth Medium (Human)

Human intestinal organoids grown in IntestiCult™-SF and IntestiCult™ Organoid Growth Medium (Human) display similar morphological characteristics during organoid maintenance and expansion. Shown are organoids from small intestinal and colonic tissues, established and expanded in IntestiCult™-SF or IntestiCult™ OGMH. Organoids are imaged at passage 5 of culture for all images. Scale bars = 500 μm.

Figure 2. IntestiCult™-SF Provides More Efficient Expansion of Intestinal Organoids Compared to IntestiCult™ OGMH

Human intestinal organoids established from both (A) small intestinal and (B) colonic tissues display more efficient expansion during extended culture when grown in IntestiCult™-SF compared to those grown in IntestiCult™ OGMH. Shown is the cumulative expansion of organoids averaged across two separate donor lines in each medium. IntestiCult™-SF demonstrates more efficient expansion of both small intestinal and colonic organoids during extended culture.

Figure 3. Organoids Grown in IntestiCult™-SF Display Some Characteristics of the Mature Intestinal Epithelium

While organoids grown in IntestiCult™-SF display a primarily "immature" phenotype including expression of (A) intestinal stem cell marker OLFM4 and (B) proliferation marker ki67. (C, D) These organoids also display some characteristics of the mature epithelium such as the presence of goblet cells (C, Muc2), enterocytes (C, KRT20), and enteroendocrine cells (D, CHGA). Scale bars = 50 μm or 20 μm as indicated.

Figure 4. Organoids Grown in IntestiCult™-SF Display Similar Genetic Expression Profiles to Those Grown in IntestiCult™ OGMH

Analysis of intestinal organoids grown in IntestiCult™-SF by qPCR of (A) Lgr5, (B) Muc2, (C) Krt20, and (D) ChgA demonstrates similar genetic expression profiles to those grown in IntestiCult™ OGMH, as well as to colonic and small intestinal tissue (A-D; x and + respectively). All expression levels are shown relative to ACTB and TBP house-keeping genes (HKGs). (E) Further analysis of three separate donors grown in IntestiCult™-SF (circles) IntestiCult™ OGMH (triangles), and published medium (squares) by principle component analysis demonstrates that differences between cultures are primarily due to donor variability with samples forming distinct clusters separated by donor, rather than by medium.

Figure 5. IntestiCult™-SF Enables Efficient Expansion of Intestinal Organoids from Polyp Tissue

Intestinal organoids were established from both normal epithelial tissue, as well as from polyp tissue, and expanded for three passages in IntestiCult™-SF. Shown are cultures from two separate donors, imaged at the end of each passage immediately before passaging.

Figure 6. Organoids Grown in IntestiCult™-SF can be Further Differentiated using IntestiCult™ Organoid Differentiation Medium

Further differentiation of organoids in IntestiCult™-SF can be achieved by passaging organoid cultures in IntestiCult™ Organoid Differentiation Medium as organoid-derived monolayers (2D Monolayer Diff) or in 3D organoid culture (3D Organoid Diff). Upon differentiation, markers for enterocytes (KRT20, ApoB), goblet cells (Muc2), and enteroendocrine cells (ChgA) are upregulated compared to organoids grown in IntestiCult™-SF.