In this webinar, Kimberly Snyder, a Senior Scientist in Pluripotent Stem Cell Biology at STEMCELL Technologies, explores the critical relationship between culture methods and the long-term genetic stability of human pluripotent stem cells (hPSCs). Aggregate-based passaging, when paired with best practices like highly characterized cell banks and standardized protocols, results in remarkably low rates of genetic abnormalities. However, single-cell passaging of hPSCs offers a scalable solution well-suited for integration into automated systems.

Kimberly presents the rationale, design, and outcomes of a large-scale, automated study evaluating the genetic stability of hPSCs under single-cell passaging conditions. Highlighting the development of eTeSR™, a culture medium specifically optimized to mitigate the risks associated with single-cell passaging, she outlines a high-throughput experiment involving 288 samples and 72 clonal lines. eTeSR™ was found to significantly reduce the occurrence of de novo genetic aberrations compared to other commercially available media, with chromosomal stability maintained in over 75% of clones during 20 weeks of single-cell culture.

As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR.