In this webinar, Robert Judson, Associate Director of Pluripotent Stem Cell Biology at STEMCELL Technologies, introduces a novel mRNA-LNP-based platform for rapidly generating forebrain neurons from human pluripotent stem cells (hPSCs). By leveraging NGN2 mRNA delivered via lipid nanoparticles, this streamlined approach offers a highly efficient and non-integrating alternative to traditional viral or transgenic overexpression systems. Compatible with multiple hPSC lines, STEMdiff™-TF Forebrain Induced Neuron Kit enables researchers to generate functional Tuj1+ neurons in just five days.

Watch this webinar to explore how neurons produced using this protocol exhibit robust glutamatergic characterization, validated through transcriptomic profiling and electrophysiological data. When co-cultured with astrocytes, these neurons demonstrate enhanced firing rates and synchronized activity, making them well-suited for disease modeling, drug screening, and functional studies on MEA platforms. STEMdiff™-TF kit offers a powerful and accessible solution for generating high-quality neurons for a range of neuroscience applications.

As a supporter of, and participant in, ISSCR’s 2025 Annual Meeting, we share with you our presentation at ISSCR 2025. This presentation does not represent an endorsement from or support of the ISSCR.