Genetic instability in human pluripotent stem cell (hPSC) cultures remains a substantial concern for research and therapeutic development outcomes, especially when cells are exposed to stressful culture conditions such as long-term single-cell passaging. In this presentation, STEMCELL Technologies’ Senior Scientist Adam Hirst introduces eTeSR™, an hPSC maintenance medium specifically developed to preserve genetic stability and cell quality during routine single-cell passaging. The formulation is optimized to support hPSC expansion while reducing cellular stress and has been tested using large-scale, long-term genetic stability studies across multiple hPSC lines. When compared against other commercially available media, the data demonstrate that hPSCs cultured in eTeSR™ display stable morphology, preserved pluripotency and trilineage differentiation, and higher genetic stability.

As a sponsor of, and participant in, ISSCR’s 2023 Annual Meeting, we share with you our Innovation Showcase presentation at #ISSCR2023. This presentation does not represent an endorsement from or support of the ISSCR.

Key topics covered in this webinar:

• • Genetic instability as a challenge in hPSC culture
• • Increased popularity of single-cell passaging in hPSC workflows
• • Development and use of eTeSR™ hPSC maintenance medium
• • Culture performance and quality characterization in eTeSR™
• • Gene editing and cloning compatibility with eTeSR™
• • Comparative, large-scale assessment of long-term genetic stability in single-cell hPSC cultures

Takeaway:
Long-term single-cell passaging places hPSCs under significant selective pressure, increasing the risk of recurrent genetic abnormalities that can affect research outcomes. By using eTeSR™, a medium specifically designed for hPSCs maintained as single-cell cultures, researchers can preserve genetic stability to achieve more reliable, reproducible gene editing, clonal expansion, and downstream differentiation workflows.