EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #04436_C
含重组人细胞因子的无血清甲基纤维素培养基
若您需要咨询产品或有任何技术问题，请通过官方电话 400 885 9050 或邮箱 info.cn@stemcell.com 与我们联系
总览
MethoCult™ SF H4436是一种无血清甲基纤维素完全培养基，用于在人骨髓、动员外周血、外周血和脐带血样本的集落形成单位（CFU）分析中培养和计数造血祖细胞。MethoCult™SF H4436无血清且成分确定，可支持红系祖细胞（BFU-E和CFU-E）、粒细胞-巨噬细胞祖细胞（CFU-GM、CFU-G和CFU-M）以及多能粒细胞、红细胞、巨噬细胞、巨核细胞祖细胞（CFU-GEMM）最佳生长。点击此处了解有关进行CFU检测的常见问题（FAQ），以及其在细胞治疗研究中的应用。
包含
• 含甲基纤维素的Iscove's MDM
• 牛血清白蛋白
• 2-巯基乙醇
• 重组人胰岛素
• 人转铁蛋白（铁饱和）
• 重组人干细胞因子 (SCF)
• 重组人白细胞介素3 (IL-3)
• 重组人白细胞介素6 (IL-6)
• 重组人促红细胞生成素 (EPO)
• 重组人粒细胞集落刺激因子 (G-CSF)
• 重组人粒细胞-巨噬细胞集落刺激因子 (GM-CSF)
• 添加物
分类
半固体培养基，专用培养基
细胞类型
造血干/祖细胞
种属
人
应用
细胞培养，克隆筛选，功能学筛选
品牌
MethoCult
研究领域
干细胞生物学
制剂类别
无血清
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
应用领域
本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。
相关材料与文献
技术资料 (4)
常见问题
文献 (15)
Tenascin C promotes hematoendothelial development and T lymphoid commitment from human pluripotent stem cells in chemically defined conditions Stem Cell Reports 2014 DEC
Abstract
The recent identification of hemogenic endothelium (HE) in human pluripotent stem cell (hPSC) cultures presents opportunities to investigate signaling pathways that are essential for blood development from endothelium and provides an exploratory platform for de novo generation of hematopoietic stem cells (HSCs). However, the use of poorly defined human or animal components limits the utility of the current differentiation systems for studying specific growth factors required for HE induction and manufacturing clinical-grade therapeutic blood cells. Here, we identified chemically defined conditions required to produce HE from hPSCs growing in Essential 8 (E8) medium and showed that Tenascin C (TenC), an extracellular matrix protein associated with HSC niches, strongly promotes HE and definitive hematopoiesis in this system. hPSCs differentiated in chemically defined conditions undergo stages of development similar to those previously described in hPSCs cocultured on OP9 feeders, including the formation of VE-Cadherin(+)CD73(-)CD235a/CD43(-) HE and hematopoietic progenitors with myeloid and T lymphoid potential.
3D microcarrier system for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum [corrected]. Regenerative medicine 2013 JUL
Abstract
BACKGROUND Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and scalable system for large scale production of hESCs and their differentiated derivatives is required. MATERIALS & METHODS hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. RESULTS hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D system can also be adapted to human induced pluripotent stem cells, which generate functional hemangioblasts with high efficiency. CONCLUSION This 3D serum- and stromal-free microcarrier system is important for future clinical applications, with the potential of developing to a GMP-compatible scalable system.
APELIN promotes hematopoiesis from human embryonic stem cells. Blood 2012 JUN
Abstract
Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendoderm populations and were enriched in hemangioblast colony-forming cells (Bl-CFC). The addition of APLN peptide to the media enhanced the growth of embryoid bodies (EBs), increased the expression of hematoendothelial genes in differentiating hESCs, and increased the frequency of Bl-CFCs by up to 10-fold. Furthermore, APLN peptide also synergized with VEGF to promote the growth of hESC-derived endothelial cells. These studies identified APLN as a novel growth factor for hESC-derived hematopoietic and endothelial cells.
更多信息
| 种属 | Human |
|---|---|
| Contains | • Methylcellulose in Iscove's MDM • Bovine serum albumin • 2-Mercaptoethanol • Recombinant human insulin • Human transferrin (iron-saturated) • Recombinant human stem cell factor (SCF) • Recombinant human interleukin 3 (IL-3) • Recombinant human interleuk |
| 配方类别 | Serum-Free |
相关产品
-
MethoCult™SF H4636含重组细胞因子的无血清甲基纤维素培养基，用于人胚胎干细胞和iPS细胞来源的细胞
-
35 mm培养皿35 mm培养皿
-
16号钝针16号鲁尔锁针头
-
3 mL注射器用于精确分装甲基纤维素培养基
