EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #100-2067_C
Noggin，无动物成分
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总览
Noggin可结合并拮抗转化生长因子β (TGF-β)家族的骨形态发生蛋白（BMP）配体。研究表明，Noggin可在小鼠胚胎成纤维细胞（MEF）条件培养基中支持未分化的人胚胎干细胞的维持培养（Chaturvedi等），并促进胚胎干细胞向多巴胺能神经元的分化及其后续存活（Chiba 等人）。Noggin 对神经管、牙齿、毛囊、眼睛等外胚层结构的发育以及中胚层体和骨骼结构的形成至关重要。此外，其还影响软骨形成、成骨和关节形成（Krause等）。本产品不含动物成分（ACF）。
亚型
细胞因子
别名
NOG，SYM1，SYNS1
细胞类型
神经干/祖细胞，神经元，多能干细胞
种属
小鼠，大鼠
研究领域
神经科学，干细胞生物学
制剂类别
Animal Component-Free
纯度
SDS-PAGE 检测：≥ 98%
实验数据
Figure 1. Biological Activity and Molecular Mass of Mouse/Rat Recombinant Noggin, ACF
(A) The biological activity of Mouse/Rat Recombinant Noggin, ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells using a luciferase reporter assay. Firefly luciferase activity was normalized to the control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 2.1 nM.
(B) 3 μg of Mouse/Rat Recombinant Noggin, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse/Rat Recombinant Noggin, ACF has a predicted molecular mass of 46 kDa (dimer). Noggin has an unusual migration pattern under non-reducing conditions due to the non-covalent dimer which is the active protein.
产品说明书及文档
请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mouse/Rat Recombinant Noggin, ACF Catalog #
100-2068 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Mouse/Rat Recombinant Noggin, ACF Catalog #
100-2068 Lot #
All Language
English Request Pricing
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更多信息
| 种属 | Mouse, Rat |
|---|---|
| 配方类别 | Animal Component-Free |
| Alternative Names | NOG, SYM1, SYNS1 |
| 纯度 | ≥ 98% by SDS-PAGE |
质量保证:
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
产品仅供研究使用，不用于针对人或动物的诊断或治疗。
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