MyoCult™ 扩增添加物 10X（小鼠）

小鼠骨骼肌祖细胞（卫星细胞）扩增的含血清补充物

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产品号 #05985_C

小鼠骨骼肌祖细胞（卫星细胞）扩增的含血清补充物

产品优势

支持小鼠骨骼肌祖细胞的高效扩增
扩增培养后的骨骼肌祖细胞保留良好的分化潜能
严格的原材料筛选与质量控制流程，有效降低批次间差异，确保实验一致性

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DMEM/F-12 with 15 mM HEPES
Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

总览

实验数据

产品说明书及文档

应用领域

总览

MyoCult™扩增添加物10X（小鼠）需与DMEM/F-12联合使用，以配制成MyoCult™扩增培养基，用于培养小鼠骨骼肌祖细胞（卫星细胞）。

该扩增培养基经过优化，适用于扩增经流式细胞分选（FACS）分离的小鼠骨骼肌卫星细胞，也适用于悬浮培养单根肌纤维。在MyoCult™扩增培养基中培养的卫星细胞可进一步分化为多核肌管细胞。

在配制MyoCult™扩增培养基时，除了使用MyoCult™扩增添加物10X，还需加入含15 mM HEPES的DMEM/F-12（产品号 #36254）。

此外，为实现卫星细胞的高效扩增，推荐使用Corning Matrigel®基质（Corning 产品号 #356234）包被的培养皿。

在配制MyoCult™扩增培养基时，除了使用MyoCult™扩增添加物10X，还需加入含15 mM HEPES的DMEM/F-12（产品号 #36254）。

此外，为实现卫星细胞的高效扩增，推荐使用Corning Matrigel®基质（Corning 产品号 #356234）包被的培养皿。

在配制MyoCult™扩增培养基时，除了使用MyoCult™扩增添加物10X，还需加入含15 mM HEPES的DMEM/F-12（产品号 #36254）。

此外，为实现卫星细胞的高效扩增，推荐使用Corning Matrigel®基质（Corning 产品号 #356234）包被的培养皿。

在配制MyoCult™扩增培养基时，除了使用MyoCult™扩增添加物10X，还需加入含15 mM HEPES的DMEM/F-12（产品号 #36254）。

此外，为实现卫星细胞的高效扩增，推荐使用Corning Matrigel®基质（Corning 产品号 #356234）包被的培养皿。

亚型
专用培养基

细胞类型
肌源干/祖细胞

种属
小鼠

应用
细胞培养，扩增

品牌
MyoCult

研究领域
干细胞生物学

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实验数据

Figure 1. FACS-Isolated Pax7+ Mouse Satellite Cells Cultured in MyoCult™ Expansion Medium Expand More Efficiently Than in Homemade Medium

FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were seeded at 2000 cells/well (24-well plate) and culture-expanded using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium. Following 6 days of culture, (A) satellite cells were immunostained for nuclei (DAPI, blue) and Pax7 (red). Also, (B) total number and (C) percentage of Pax7+ satellite cells were quantified (n = 3). Error bars represent standard error of mean (SEM). Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.

Figure 2. FACS-Isolated Mouse Satellite Cells Are Expandable Over Multiple Passages in Myocult™ Expansion Medium

FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were seeded at 2000 cells/well (24-well plate) and cultured using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium. Following 12 days of expansion (2 passages), (A) satellite cells were imaged using phase contrast microscopy and (B) total numbers of MyoD+ satellite cells were quantified (n = 3). Error bars represent SEM. Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.

Figure 3. Mouse Satellite Cells Expanded in Myocult™ Expansion Medium Maintain Differentiation Capacity

FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were culture-expanded using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium for 2 passages (12 days following FACS isolation) and then treated with differentiation medium (high glucose DMEM with 2% Horse Serum). Following 4 days of differentiation, (A) myotubes cultured in MyoCult™ Expansion Medium were immunostained for nuclei (DAPI, blue) and Myosin Heavy Chain (MyHC, red), and (B) percentage of nuclei localized within MyHC+ myotubes (fusion index) was quantified (n = 3). Error bars represent SEM. Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.

Figure 4. Mouse Single Isolated Myofibers Can Be Cultured in MyoCult™ Expansion Medium Without Requiring Additional Supplements

Single isolated myofibers were cultured in suspension using complete MyoCult™ Expansion Medium (Mouse) or a homemade myofiber culture medium. After 4 days, (A) intact, viable myofibers were quantified (n = 3) and (B) immunostained for Pax7 (Red). Error bars represent SEM. Myofiber homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia. A specific myofiber medium (or additional supplements) is not needed for myofiber culture when using MyoCult™ Expansion Medium.