NeuroFluor™NeuO

用于检测活神经元的透膜荧光探针

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产品号 #（选择产品）

产品号 #01801_C

用于检测活神经元的透膜荧光探针

产品优势

使选择性标记小鼠，大鼠，或人类神经元不固定
能否证实人多能干细胞衍生神经祖细胞的神经分化
能在其他脑细胞存在的情况下用来标记神经元吗
无毒，非永久性
简单快速的标记方案

总览

实验数据

产品说明书及文档

应用领域

总览

NeuroFluor™ NeuO 是一种具有膜通透性的荧光探针，可在存在其他脑细胞的环境中，选择性标记原代神经元以及由多能干细胞分化而来的神经元 (JC Er et al. Angew Chem Int Ed, 2015)。使用该探针染色的细胞可通过荧光成像直接可视化。值得注意的是，NeuroFluor™ NeuO 并不会与细胞永久结合，其染色是可逆的，可通过洗涤去除染料，从而保留未标记的活细胞以用于后续实验操作。有关更多应用信息，请参阅相关文献。

包含
• 100 µM NeuO（CAS编号：1616355-50-0，1668597-38-3） • DMSO

细胞类型
神经细胞，PSC衍生，神经元

种属
人，小鼠，大鼠

应用
鉴定，表型鉴定

品牌
NeuroFluor

研究领域
疾病建模，神经科学，干细胞生物学

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实验数据

Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.