EasySep™小鼠TIL（CD45）正选试剂盒
产品号 #07154_C
用于胚胎干细胞和诱导多能干细胞分化的细胞培养添加物
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总览
烟酰胺（NAM）是一种用于胚胎干细胞（ESC）和诱导多能干细胞（iPSC）分化的细胞培养添加物。它是维生素B3的酰胺衍生物，是聚（ADP -核糖）聚合酶（PARP）抑制剂，也是NAD+的主要前体。烟酰胺已被证明可在各种应用中调节干细胞分化，其中最显著的是调节小鼠胰腺ESC和iPSC向胰岛样胰岛素分泌细胞进行分化。
细胞类型
内胚层，PSC衍生，胰腺细胞，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化，扩增
研究领域
上皮细胞研究，干细胞生物学
CAS 编号
98-92-0
产品说明书及文档
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应用领域
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相关材料与文献
技术资料 (1)
文献 (1)
Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. The Journal of clinical investigation 1993 SEP
Abstract
The effects of nicotinamide (NIC) on human fetal and adult endocrine pancreatic cells were studied in tissue culture. Treatment of the fetal cells with 10 mM NIC resulted in a twofold increase in DNA content and a threefold increase in insulin content. This was associated with the development of beta cell outgrowths from undifferentiated epithelial cell clusters and an increase in the expression of the insulin, glucagon, and somatostatin genes. DNA synthesis was stimulated only in the undifferentiated cells. Half-maximal doses for the insulinotropic and mitogenic effects of NIC were 5-10 and 1-2 mM, respectively. Islet-like cell clusters cultured with NIC responded to glucose stimulation with a biphasic increase in insulin release (fourfold peak), whereas control cells were unresponsive to glucose. Both control and NIC-treated cells developed into functional islet tissue after transplantation into athymic nude mice. As compared with adult islets, the insulinotropic action of NIC could only be demonstrated in the fetal cells. Our results indicate that NIC induces differentiation and maturation of human fetal pancreatic islet cells. This model should be useful for the study of molecular mechanisms involved in beta cell development.
更多信息
| 种属 | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 98-92-0 |
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