STEMCELL’s portfolio of TeSR™ 3D-optimized media products have been developed for robust and scalable suspension culture of human pluripotent stem cells (hPSCs) in aggregates. The workflows include non-enzymatic dissociation using the Gentle Cell Dissociation Reagent and filter-based trituration. Watch this technical video to learn how to passage a 2 mL hPSC suspension culture in a 6-well plate.