PBS-MINI MagDrive生物反应器

紧凑型生物反应器，配备一次性容器，用于hPSC和其他细胞类型的高通量3D悬浮培养

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产品号 #100-1005_C

紧凑型生物反应器，配备一次性容器，用于hPSC和其他细胞类型的高通量3D悬浮培养

产品优势

紧凑。紧凑的不锈钢基座可灵活放置于不同的实验区域，包括超净工作台。
安全高效的扩增。Vertical-Wheel™叶轮和U形底座营造出低剪切混合环境，非常适合培养大小均匀的hPSC聚集体。
易于回收聚集体。U型底座可以回收聚集体，宽直径的盖子可以方便地回收聚集体。
完美搭配。基座设计用于0.1 L和0.5 L PBS-MINI MAG一次性培养容器，通过MagDrive技术对容器进行磁力驱动。

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总览

实验数据

产品说明书及文档

应用领域

总览

使用PBS-MINI MagDrive生物反应器基座和MAG一次性培养容器，可靠快速地放大用户的3D细胞培养和悬浮液规模。Vertical-Wheel™搅拌器提供温和而高效的搅拌，无需使用消泡剂或剪切保护剂即可扩增对剪切敏感的细胞。紧凑、密封的基座适用于人多能干细胞（hPSC）培养，且可在培养箱内使用。搭配便捷操作旋钮和数字显示屏可方便地控制用户的培养系统，并使用内置LED灯在弱光条件下观察细胞培养状态。

PBS-MINI 0.1升和0.5升 MAG一次性培养容器可单独购买，每包4个。这些一次性容器不含动物成分，符合USP VI 级塑料检测<88>和 ISO 10993 的要求。PBS Biotech Inc. 为PBS-MINI MagDrive 生物反应器基座提供18个月的保修，从发货之日起有效。本产品仅供研究使用。

PBS-MINI MagDrive生物反应器基座包含一个电源适配器，兼容A型电源插座和120V或240V交流输入。在北美以外地区使用时需另行购买壁式适配器；如需购买特定地区的电源线，请联系info.cn@stemcell.com.

应用
细胞培养，扩增

品牌
PBS-MINI

研究领域
干细胞生物学

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实验数据

Figure 1. Consistently High Daily Fold Expansion of Human Pluripotent Stem Cells (hPSCs) Is Achieved with the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

Daily fold expansions of a human embryonic stem (ES) cell line (H9) and human induced pluripotent stem (iPS) cell line (WLS-1C), cultured in PBS-MINI (A) 0.1 and (B) 0.5 MAG Single-Use Vessels, show consistently high growth. The cells were seeded at a density of 8 x 10⁴ viable cells/mL in 100 mL (for PBS-MINI 0.1 MAG) and 3 - 8 x 10⁴ viable cells/mL in 500 mL (for PBS-MINI 0.5 MAG), and cultured for 4 days using mTeSR™3D (Catalog #03950). Fold expansion of each cell line was comparable to their growth in Nalgene™ filter bottles (Control), when seeded at a density of 1 x 10⁵ viable cells/mL in 30 mL. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

Figure 2. Culturing hPSCs in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Achieves High Cell Viability

End-of-passage viabilities of a human ES cell line (H9) and human iPS cell line (WLS-1C) cultured in mTeSR™3D show that the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels support expansion of hPSCs at (A) 100 mL (with PBS-MINI 0.1 MAG) and (B) 500 mL (with PBS-MINI 0.5 MAG) culture volumes. Error bars are +/- SD (n = 3 for PBS-MINI 0.1 and 0.5 conditions, and n = 2 for control).

Figure 3. Markers of Undifferentiated hPSCs Harvested from the PBS-MINI 0.5 MAG Single-Use Vessels

1C and H9 hPSC lines after 9 passages in the PBS-MINI 0.5 MAG Single-Use Vessels using mTeSR™3D show high OCT-4 and TRA-1-60 expression. Error bars are +/- SD (n = 1).

Figure 4. Morphology of hPSC Aggregates Is Optimal When Using the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels

hPSCs, expanded in suspension using mTeSR™3D in the PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels, exhibit the typical morphological characteristics of hPSC aggregates in suspension culture, including a mostly spherical shape, clear edges (not smooth or shiny), an even color, and a dimpled or pock-marked appearance. Aggregates are typically 350-400 µm in size. Scale bar = 500 µm.

Figure 5. PBS-MINI 0.1 and 0.5 MAG Single-Use Vessels Demonstrate Consistently High Daily Fold Expansion Compared to Common Alternative Culture Vessels

Daily fold expansion of 7 cell lines in suspension culture vessels, tested from 2019 - 2022. Of the 9 vessels tested, those recommended for robust suspension culture scale-up are the Non Tissue Culture-Treated 6-Well Plate, Nalgene™ Filter Storage Bottle, PBS-MINI 0.1, and PBS-MINI 0.5. These vessels have a consistent mean daily fold expansion above 1.2.