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鬼笔环肽iFluor™偶联物

适用于细胞培养中F-肌动蛋白检测的荧光染料偶联物
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产品号 #100-0852_C

适用于细胞培养中F-肌动蛋白检测的荧光染料偶联物

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实验数据
产品说明书及文档
应用领域
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总览

鬼笔环肽是一种七肽，来源于有毒真菌毒蝇伞（Amanita phalloides）。鬼笔环肽可高特异性地结合细胞内的F-肌动蛋白（F-actin）丝，并可防止其去聚合。荧光标记的鬼笔环肽可用于对经甲醛固定并通透处理后的细胞中F-actin的标记、识别与定量分析。与大多数动植物物种的肌动蛋白抗体相比，荧光鬼笔环肽表现出更低的非特异性结合，从而在成像过程中提供较低背景信号和更高图像对比度。

细胞类型
神经细胞，PSC衍生，神经元，多能干细胞
 
应用
鉴定
 
研究领域
癌症，神经科学，干细胞生物学
 
CAS 编号
67-68-5
 
分子量
鬼笔环肽，iFluor™ 488*: 1399.49 克/摩尔; 鬼笔环肽，iFluor™ 555*: 1468.53 克/摩尔; 鬼笔环肽，iFluor™ 647: 1632.87 克/摩尔
 

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实验数据

Image of H9 embryonic stem cells stained with Phalloidin-iFluor™ 647.

Figure 1. Phalloidin-iFluor™ Can Be Used to Stain Embryonic Stem Cells

H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).

Image of forebrain-type neurons stained with Phalloidin-iFluor™ 647.

Figure 2. Phalloidin-iFluor™ Can Be Used to Stain Forebrain-Type Neurons

WLS-1C cell-derived neural progenitor cells were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kits (Catalog #08605). Neurons were cultured on poly-L-ornithine/laminin-coated 35 mm glass-bottom dishes for 3 weeks, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).

产品说明书及文档

请在《产品说明书》中查找相关支持信息和使用说明，或浏览下方更多实验方案。

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English

应用领域

本产品专为以下研究领域设计，适用于工作流程中的高亮阶段。探索这些工作流程，了解更多我们为各研究领域提供的其他配套产品。

Research Area
Workflow Stages

更多信息

更多信息
Molecular Weight Phalloidin, iFluor™ 488*: 1399.49 g/mol; Phalloidin, iFluor™ 555*: 1468.53 g/mol; Phalloidin, iFluor™ 647: 1632.87 g/mol
Cas Number 67-68-5
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