This video provides a step-by-step procedure for setting up hematopoietic colony-forming unit (CFU) assays using semi-solid methylcellulose-based MethoCult™ medium. This procedure includes preparing materials and cells, plating in MethoCult™ medium, and incubating. 0:13 - Preparing MethoCult™ Medium 1:31 - Preparing Cells 3:02 - Adding Cells to MethoCult™ Medium 3:37 - Plating and Incubating 5:14 - Analysis of CFU Assays Learn more about MethoCult™ > Learn more about accurately identifying and counting colonies in the human hematopoietic CFU assay by registering for our Online CFU Assay Course.